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Synthetic RNA-based logic computation in mammalian cells

Matsuura, Satoshi ; Ono, Hiroki ; Kawasaki, Shunsuke ; Kuang, Yi ; Fujita, Yoshihiko ; Saito, Hirohide

Nature communications, 2018-11, Vol.9 (1), p.4847-8, Article 4847 [Periódico revisado por pares]

England: Nature Publishing Group

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Título:
Synthetic RNA-based logic computation in mammalian cells
Autor: Matsuura, Satoshi ; Ono, Hiroki ; Kawasaki, Shunsuke ; Kuang, Yi ; Fujita, Yoshihiko ; Saito, Hirohide
Assuntos: Apoptosis ; Circuit design ; Circuits ; Computation ; Decision making ; Deoxyribonucleic acid ; Design ; DNA ; Gates (circuits) ; Gene expression ; Logic ; Logic circuits ; Mammalian cells ; Mammals ; MicroRNAs ; miRNA ; mRNA ; Protein expression ; Proteins ; Ribonucleic acid ; RNA ; RNA-binding protein ; Therapeutic applications ; Transgenes
É parte de: Nature communications, 2018-11, Vol.9 (1), p.4847-8, Article 4847
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Synthetic biological circuits are designed to regulate gene expressions to control cell function. To date, these circuits often use DNA-delivery methods, which may lead to random genomic integration. To lower this risk, an all RNA system, in which the circuit and delivery method are constituted of RNA components, is preferred. However, the construction of complexed circuits using RNA-delivered devices in living cells has remained a challenge. Here we show synthetic mRNA-delivered circuits with RNA-binding proteins for logic computation in mammalian cells. We create a set of logic circuits (AND, OR, NAND, NOR, and XOR gates) using microRNA (miRNA)- and protein-responsive mRNAs as decision-making controllers that are used to express transgenes in response to intracellular inputs. Importantly, we demonstrate that an apoptosis-regulatory AND gate that senses two miRNAs can selectively eliminate target cells. Thus, our synthetic RNA circuits with logic operation could provide a powerful tool for future therapeutic applications.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Synthetic RNA-based logic computation in mammalian cells

Matsuura, Satoshi ; Ono, Hiroki ; Kawasaki, Shunsuke ; Kuang, Yi ; Fujita, Yoshihiko ; Saito, Hirohide

England: Nature Publishing Group

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