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The expression of the surfactant proteins SP-A and SP-B during postnatal alveolarization of the rat lung

Roeder, Franziska ; Knudsen, Lars ; Schmiedl, Andreas

PloS one, 2024-03, Vol.19 (3), p.e0297889-e0297889 [Periódico revisado por pares]

United States: Public Library of Science

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  • Título:
    The expression of the surfactant proteins SP-A and SP-B during postnatal alveolarization of the rat lung
  • Autor: Roeder, Franziska ; Knudsen, Lars ; Schmiedl, Andreas
  • Assuntos: Alveolar process ; Analysis ; Animals ; Biology and Life Sciences ; Evaluation ; Gene expression ; Genes ; Lipoproteins - metabolism ; Lung - metabolism ; Medicine and Health Sciences ; Physical Sciences ; Proteins ; Pulmonary Alveoli ; Pulmonary Surfactant-Associated Proteins - genetics ; Pulmonary Surfactant-Associated Proteins - metabolism ; Pulmonary Surfactants - metabolism ; Rats ; Research and Analysis Methods ; Surface active agents ; Surface-Active Agents - metabolism ; Wright, J.R
  • É parte de: PloS one, 2024-03, Vol.19 (3), p.e0297889-e0297889
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    Competing Interests: The authors have declared that no competing interests exist.
  • Descrição: Surfactant-specific proteins (SP) are responsible for the functional and structural integrity as well as for the stabilization of the intra-alveolar surfactant. Morphological lung maturation starts in rat lungs after birth. The aim of this study was to investigate whether the expression of the hydrophilic SP-A and the hydrophobic SP-B is associated with characteristic postnatal changes characterizing morphological lung maturation. Stereological methods were performed on the light microscope. Using immunohistochemical and molecular biological methods (Western Blot, RT-qPCR), the SP-A and SP-B of adult rat lungs and of those with different postnatal developmental stages (3, 7, 14 and 21 days after birth) were characterized. As signs of alveolarization the total septal surface and volume increased and the septal thickness decreased. The significantly highest relative surface fraction of SP-A labeled alveolar epithelial cells type II (AEII) was found together with the highest relative SP-A gene expression before the alveolarization (3th postnatal day). With the downregulation of SP-A gene expression during and after alveolarization (between postnatal days 7 and 14), the surface fraction of the SP-A labeled AEII also decreased, so they are lowest in adult animals. The surface fraction of SP-B labeled AEII and the SP-B gene expression showed the significantly highest levels in adults, the protein expression increased also significantly at the end of morphological lung maturation. There were no alterations in the SP-B expression before and during alveolarization until postnatal day 14. The protein expression as well as the gene expression of SP-A and SP-B correlated very well with the total surface of alveolar septa independent of the postnatal age. The expression of SP-A and SP-B is differentially associated with morphological lung maturation and correlates with increased septation of alveoli as indirect clue for alveolarization.
  • Editor: United States: Public Library of Science
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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