Idioma:

Gellan gum: A new biomaterial for cartilage tissue engineering applications

Oliveira, J. T. ; Martins, L. ; Picciochi, R. ; Malafaya, P. B. ; Sousa, R. A. ; Neves, N. M. ; Mano, J. F. ; Reis, R. L.

Journal of biomedical materials research. Part A, 2010-06, Vol.93A (3), p.852-863 [Periódico revisado por pares]

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Texto completo disponível

Citações Citado por

Enviar para

Título:
Gellan gum: A new biomaterial for cartilage tissue engineering applications
Autor: Oliveira, J. T. ; Martins, L. ; Picciochi, R. ; Malafaya, P. B. ; Sousa, R. A. ; Neves, N. M. ; Mano, J. F. ; Reis, R. L.
Assuntos: Adult ; Aged ; Animals ; Biocompatible Materials - pharmacology ; Biological and medical sciences ; Biotechnology ; cartilage ; Cartilage - drug effects ; Cartilage - physiology ; Cell Death - drug effects ; Cell Line ; Cell Survival - drug effects ; Chondrocytes - cytology ; Chondrocytes - drug effects ; Fundamental and applied biological sciences. Psychology ; Health. Pharmaceutical industry ; Humans ; hydrogel ; Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology ; Industrial applications and implications. Economical aspects ; Mechanical Phenomena - drug effects ; Medical sciences ; Membranes, Artificial ; Middle Aged ; Miscellaneous ; natural origin ; Phase Transition - drug effects ; polysaccharide ; Polysaccharides, Bacterial - pharmacology ; Polysaccharides, Bacterial - ultrastructure ; Rats ; Rheology - drug effects ; Sepharose - pharmacology ; Sphingomonas paucimobilis ; Staining and Labeling ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments ; tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds
É parte de: Journal of biomedical materials research. Part A, 2010-06, Vol.93A (3), p.852-863
Notas: ark:/67375/WNG-882M3WHS-4
istex:77EF7E717EC6DD96C8F6A3644CB91CE428279329
The European Project HIPPOCRATES - No. STRP 505758-1
The Portuguese Foundation for Science and Technology (FCT) - No. SFRH/BD17135/2004
The European NoE EXPERTISSUES - No. NMP3-CT-2004-500283
ArticleID:JBM32574
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Gellan gum is a polysaccharide manufactured by microbial fermentation of the Sphingomonas paucimobilis microorganism, being commonly used in the food and pharmaceutical industry. It can be dissolved in water, and when heated and mixed with mono or divalent cations, forms a gel upon lowering the temperature under mild conditions. In this work, gellan gum hydrogels were analyzed as cells supports in the context of cartilage regeneration. Gellan gum hydrogel discs were characterized in terms of mechanical and structural properties. Transmissionelectron microscopy revealed a quite homogeneous chain arrangement within the hydrogels matrix, and dynamic mechanical analysis allowed to characterize the hydrogels discs viscoelastic properties upon compression solicitation, being the compressive storage and loss modulus of ∼40 kPa and 3 kPa, respectively, at a frequency of 1 Hz. Rheological measurements determined the sol‐gel transition started to occur at approximately 36°C, exhibiting a gelation time of ∼11 s. Evaluation of the gellan gum hydrogels biological performance was performed using a standard MTS cytotoxicity test, which showed that the leachables released are not deleterious to the cells and hence were noncytotoxic. Gellan gum hydrogels were afterwards used to encapsulate human nasal chondrocytes (1 × 106 cells/mL) and culture them for total periods of 2 weeks. Cells viability was confirmed using confocal calcein AM staining. Histological observations revealed normal chondrocytes morphology and the obtained data supports the claim that this new biomaterial has the potential to serve as a cell support in the field of cartilage regeneration. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010
Editor: Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Gellan gum: A new biomaterial for cartilage tissue engineering applications

Oliveira, J. T. ; Martins, L. ; Picciochi, R. ; Malafaya, P. B. ; Sousa, R. A. ; Neves, N. M. ; Mano, J. F. ; Reis, R. L.

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Buscando em bases de dados remotas. Favor aguardar.