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Single‐Response Duplexing of Electrochemical Label‐Free Biosensor from the Same Tag

Costa, Juliana N. Y. ; Pimentel, Gabriel J. C. ; Poker, Júlia A. ; Merces, Leandro ; Paschoalino, Waldemir J. ; Vieira, Luis C. S. ; Castro, Ana C. H. ; Alves, Wendel A. ; Ayres, Lucas B. ; Kubota, Lauro T. ; Santhiago, Murilo ; Garcia, Carlos D. ; Piazzetta, Maria H. O. ; Gobbi, Angelo L. ; Shimizu, Flávio M. ; Lima, Renato S.

Advanced healthcare materials, 2024-04, Vol.13 (11), p.e2303509-n/a [Periódico revisado por pares]

Germany: Wiley Subscription Services, Inc

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Título:
Single‐Response Duplexing of Electrochemical Label‐Free Biosensor from the Same Tag
Autor: Costa, Juliana N. Y. ; Pimentel, Gabriel J. C. ; Poker, Júlia A. ; Merces, Leandro ; Paschoalino, Waldemir J. ; Vieira, Luis C. S. ; Castro, Ana C. H. ; Alves, Wendel A. ; Ayres, Lucas B. ; Kubota, Lauro T. ; Santhiago, Murilo ; Garcia, Carlos D. ; Piazzetta, Maria H. O. ; Gobbi, Angelo L. ; Shimizu, Flávio M. ; Lima, Renato S.
Assuntos: accuracy ; Antibodies ; Antibodies, Viral - blood ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biosensors ; COVID-19 ; COVID-19 - blood ; COVID-19 - diagnosis ; Crosstalk ; Electrochemical Techniques - instrumentation ; Electrochemical Techniques - methods ; Electrodes ; Ferricyanide ; Gold - chemistry ; Humans ; Immunoassay - instrumentation ; Immunoassay - methods ; Immunosensors ; Labels ; multiplexed detection ; Multiplexing ; SARS-CoV-2 - isolation & purification ; serology ; Silver chloride ; single‐channel potentiostat ; square wave voltammetry ; steric hindrance ; throughput
É parte de: Advanced healthcare materials, 2024-04, Vol.13 (11), p.e2303509-n/a
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SourceType-Scholarly Journals-1
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Descrição: Multiplexing is a valuable strategy to boost throughput and improve clinical accuracy. Exploiting the vertical, meshed design of reproducible and low‐cost ultra‐dense electrochemical chips, the unprecedented single‐response multiplexing of typical label‐free biosensors is reported. Using a cheap, handheld one‐channel workstation and a single redox probe, that is, ferro/ferricyanide, the recognition events taking place on two spatially resolved locations of the same working electrode can be tracked along a single voltammetry scan by collecting the electrochemical signatures of the probe in relation to different quasi‐reference electrodes, Au (0 V) and Ag/AgCl ink (+0.2 V). This spatial isolation prevents crosstalk between the redox tags and interferences over functionalization and binding steps, representing an advantage over the existing non‐spatially resolved single‐response multiplex strategies. As proof of concept, peptide‐tethered immunosensors are demonstrated to provide the duplex detection of COVID‐19 antibodies, thereby doubling the throughput while achieving 100% accuracy in serum samples. The approach is envisioned to enable broad applications in high‐throughput and multi‐analyte platforms, as it can be tailored to other biosensing devices and formats. Meshed ultra‐dense chips provide the single‐response duplexing of label‐free electrochemical biosensors. Distinguishable signatures are achieved by interrogating the same redox probe in spatially resolved areas of the same working electrode relative to distinct quasi‐reference electrodes, Au and Ag/AgCl. This spatial isolation prevents crosstalk between probes and interferences over functionalization/binding steps, representing an advantage over existing non‐spatially resolved single‐response multiplex strategies.
Editor: Germany: Wiley Subscription Services, Inc
Idioma: Inglês

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Single‐Response Duplexing of Electrochemical Label‐Free Biosensor from the Same Tag

Germany: Wiley Subscription Services, Inc

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