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Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research

Perez de Souza, Leonardo ; Alseekh, Saleh ; Scossa, Federico ; Fernie, Alisdair R

Nature methods, 2021-07, Vol.18 (7), p.733-746 [Periódico revisado por pares]

United States: Nature Publishing Group

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Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research
Autor: Perez de Souza, Leonardo ; Alseekh, Saleh ; Scossa, Federico ; Fernie, Alisdair R
Assuntos: Biological research ; Biology, Experimental ; Chromatography ; Chromatography, High Pressure Liquid - instrumentation ; Chromatography, High Pressure Liquid - methods ; High performance liquid chromatography ; High resolution ; Identification and classification ; Ion Mobility Spectrometry - instrumentation ; Ion Mobility Spectrometry - methods ; Liquid chromatography ; Magnetic Resonance Spectroscopy ; Mass spectrometry ; Mass Spectrometry - methods ; Mass spectroscopy ; Metabolites ; Metabolomics ; Metabolomics - methods ; Methods ; Scientific imaging ; Solid Phase Extraction - methods ; Spectrometry, Mass, Electrospray Ionization ; Spectroscopy
É parte de: Nature methods, 2021-07, Vol.18 (7), p.733-746
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
ObjectType-Review-1
Descrição: Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) variants currently represent the best tools to tackle the challenges of complexity and lack of comprehensive coverage of the metabolome. UHPLC offers flexible and efficient separation coupled with high-sensitivity detection via HRMS, allowing for the detection and identification of a broad range of metabolites. Here we discuss current common strategies for UHPLC-HRMS-based metabolomics, with a focus on expanding metabolome coverage.
Editor: United States: Nature Publishing Group
Idioma: Inglês

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Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research

Perez de Souza, Leonardo ; Alseekh, Saleh ; Scossa, Federico ; Fernie, Alisdair R

United States: Nature Publishing Group

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