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Release and degradation of environmental DNA and RNA in a marine system

Wood, Susanna A. ; Biessy, Laura ; Latchford, Janie L. ; Zaiko, Anastasija ; von Ammon, Ulla ; Audrezet, François ; Cristescu, Melania E. ; Pochon, Xavier

The Science of the total environment, 2020-02, Vol.704, p.135314-135314, Article 135314 [Periódico revisado por pares]

Netherlands: Elsevier B.V

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  • Título:
    Release and degradation of environmental DNA and RNA in a marine system
  • Autor: Wood, Susanna A. ; Biessy, Laura ; Latchford, Janie L. ; Zaiko, Anastasija ; von Ammon, Ulla ; Audrezet, François ; Cristescu, Melania E. ; Pochon, Xavier
  • Assuntos: Animals ; Aquatic Organisms ; Biodiversity ; Biomonitoring ; Detection ; Droplet digital PCR ; Ecosystem ; Environmental DNA ; Environmental Monitoring - methods ; Polymerase Chain Reaction ; RNA ; Species detection
  • É parte de: The Science of the total environment, 2020-02, Vol.704, p.135314-135314, Article 135314
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: [Display omitted] •The efficacies of eDNA/RNA based approaches depend on the release and degradation of these molecules.•eDNA/RNA release and degradation was investigated for two marine invertebrates using ddPCR.•eRNA persisted for longer than expected (13 h) and decay rate constants for eDNA/RNA were similar.•There was no evidence that the decay rates constants for eDNA and eRNA were different.•Using eRNA may provide new opportunities for improved biodiversity surveys and transcriptomics. Over the last decade, there has been growing interest in the analysis of environmental DNA (eDNA) to infer the presence of organisms in aquatic environments. The efficacy of eDNA/eRNA based tools are highly depend on the turnover rate of the molecule (their release and degradation). Environmental DNA has been shown to persist for days, weeks or years in environmental samples. Environmental RNA (eRNA) is thought to degrade faster than eDNA, however to our knowledge, no experimental studies have explored this. Here we present an aquarium study to investigate eDNA and eRNA shedding rates and degradation for two sessile marine invertebrates. The copy numbers for eDNA and eRNA were assessed using droplet digital PCR targeting the mitochondrial Cytochrome c Oxidase subunit 1 (COI) gene. Environmental RNA persisted after organism removal for much longer than expected with detections for up to 13 h. In contrast, eDNA was detected is samples collected up to 94 h after organism removal. There was no evidence that the decay rates constants for eDNA and eRNA were different (p = 0.6, Kruskal-Wallis tests). Both eDNA and eRNA was detected in biofilms collected at the end of the experiment (day 21). This suggests binding with organic or inorganic compounds or stabilization of these molecules in the biofilm matrix. The finding of the prolonged persistence of eRNA may provide new opportunities for improved biodiversity surveys through reducing false positives caused by legacy DNA and could also facilitate new research on environmental transcriptomics.
  • Editor: Netherlands: Elsevier B.V
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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