Idioma:

EBV-miR-BART7-3p promotes the EMT and metastasis of nasopharyngeal carcinoma cells by suppressing the tumor suppressor PTEN

Cai, L-M ; Lyu, X-M ; Luo, W-R ; Cui, X-F ; Ye, Y-F ; Yuan, C-C ; Peng, Q-X ; Wu, D-H ; Liu, T-F ; Wang, E ; Marincola, F-M ; Yao, K-T ; Fang, W-Y ; Cai, H-B ; Li, X

Oncogene, 2015-04, Vol.34 (17), p.2156-2166 [Periódico revisado por pares]

England: Nature Publishing Group

Texto completo disponível

Citações Citado por

Enviar para

Título:
EBV-miR-BART7-3p promotes the EMT and metastasis of nasopharyngeal carcinoma cells by suppressing the tumor suppressor PTEN
Autor: Cai, L-M ; Lyu, X-M ; Luo, W-R ; Cui, X-F ; Ye, Y-F ; Yuan, C-C ; Peng, Q-X ; Wu, D-H ; Liu, T-F ; Wang, E ; Marincola, F-M ; Yao, K-T ; Fang, W-Y ; Cai, H-B ; Li, X
Assuntos: 1-Phosphatidylinositol 3-kinase ; AKT protein ; Biological products ; Cancer ; Carcinoma ; Cell adhesion & migration ; Cell Line ; Cell migration ; Cell Nucleus - genetics ; Cell Nucleus - metabolism ; Cell Nucleus - pathology ; Cellular biology ; Development and progression ; Emt protein ; Epithelial-Mesenchymal Transition ; Epstein-Barr virus ; Epstein-Barr Virus Infections - genetics ; Epstein-Barr Virus Infections - metabolism ; Epstein-Barr Virus Infections - pathology ; Female ; Gene expression ; Genetic aspects ; Glycogen Synthase Kinase 3 - genetics ; Glycogen Synthase Kinase 3 - metabolism ; Glycogen Synthase Kinase 3 beta ; Health aspects ; Herpesvirus 4, Human - genetics ; Herpesvirus 4, Human - metabolism ; Humans ; Lymph nodes ; Malignancy ; Mesenchyme ; Metastases ; Metastasis ; MicroRNA ; miRNA ; Nasopharyngeal cancer ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms - genetics ; Nasopharyngeal Neoplasms - metabolism ; Nasopharyngeal Neoplasms - pathology ; Nasopharyngeal Neoplasms - virology ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Phenotypic reversion ; Phosphatidylinositol 3-Kinases - genetics ; Phosphatidylinositol 3-Kinases - metabolism ; Proto-Oncogene Proteins c-akt - genetics ; Proto-Oncogene Proteins c-akt - metabolism ; PTEN Phosphohydrolase - biosynthesis ; PTEN Phosphohydrolase - genetics ; PTEN protein ; Reversion ; RNA, Neoplasm - genetics ; RNA, Neoplasm - metabolism ; RNA, Viral - genetics ; RNA, Viral - metabolism ; Signal Transduction - genetics ; Therapeutic applications ; Tumor suppressor genes ; β-Catenin
É parte de: Oncogene, 2015-04, Vol.34 (17), p.2156-2166
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The epithelial-mesenchymal transition (EMT) is crucial to cancer progression and metastasis. Although multiple cellular miRNAs have been identified to regulate the EMT and metastasis in cancers, the role of viral miRNAs in cancer progression remains largely unknown. Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated malignancy typically characterized by its early metastasis. In the present study, we have discovered the involvement of a viral miRNA, EBV-miR-BART7-3p, in the EMT and metastasis of NPC cells. Initially, we observed that EBV-miR-BART7-3p was highly expressed in NPC and positively correlated with lymph node metastasis and clinical stage of NPC. Subsequently, we demonstrated that EBV-miR-BART7-3p enhanced cell migration/invasion in vitro, cancer metastasis in vivo, and particularly the EMT characterized by loss of epithelial markers and gain of mesenchymal features in NPC cells. Furthermore, mechanistic studies disclosed that EBV-miR-BART7-3p targeted a major human tumor suppressor PTEN, modulating PI3K/Akt/GSK-3β signaling and eventually leading to the high expression and nuclear accumulation of Snail and β-catenin, which favor EMT. Knockdown of PTEN could phenocopy the effect of EBV-miR-BART7-3p, whereas re-expression of PTEN resulted in a phenotypic reversion. Moreover, these findings were supported by an observation of an EBV-positive cell model in which silencing of endogenous EBV-miR-BART7-3p partially attenuated cell migration/invasion and altered EMT protein expression pattern via reverting PI3K/Akt, Snail and β-catenin expression. Thus, this study suggests a novel mechanism by which EBV-miR-BART7-3p modulates the EMT and metastasis of NPC cells, and a clinical implication of EBV-miR-BART7-3p as a potential biomarker or therapeutic target.
Editor: England: Nature Publishing Group
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

EBV-miR-BART7-3p promotes the EMT and metastasis of nasopharyngeal carcinoma cells by suppressing the tumor suppressor PTEN

Cai, L-M ; Lyu, X-M ; Luo, W-R ; Cui, X-F ; Ye, Y-F ; Yuan, C-C ; Peng, Q-X ; Wu, D-H ; Liu, T-F ; Wang, E ; Marincola, F-M ; Yao, K-T ; Fang, W-Y ; Cai, H-B ; Li, X

England: Nature Publishing Group

Buscando em bases de dados remotas. Favor aguardar.