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Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda

Carvalho, Renato A ; Omoto, Celso ; Field, Linda M ; Williamson, Martin S ; Bass, Chris Vontas, John

PloS one, 2013-04, Vol.8 (4), p.e62268 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda
Autor: Carvalho, Renato A ; Omoto, Celso ; Field, Linda M ; Williamson, Martin S ; Bass, Chris
Vontas, John
Assuntos: Acetylcholinesterase ; Acetylcholinesterase - metabolism ; Acids ; Agriculture ; Agrochemicals ; Amino Acid Sequence ; Amino acids ; Animals ; Bioassays ; Biological Assay ; Biological assays ; Biology ; Chemistry ; Chlorpyrifos ; Chlorpyrifos - toxicity ; Corn ; Cyhalothrin ; Cytochrome ; Detoxification ; DNA microarrays ; DNA, Complementary - genetics ; Enzyme inhibitors ; Enzymes ; Expressed Sequence Tags ; Gene amplification ; Gene expression ; Gene Expression Regulation - drug effects ; Gene families ; Gene sequencing ; Genes ; Genotype ; Glutathione ; Grain ; Grain crops ; Insecticide resistance ; Insecticide Resistance - drug effects ; Insecticide Resistance - genetics ; Insecticides ; Insects ; Investigations ; Laboratories ; Lepidoptera ; Metabolism ; Molecular modelling ; Molecular Sequence Annotation ; Molecular Sequence Data ; Mortality ; Musca domestica ; Mutation ; Mutation - genetics ; Myzus persicae ; Nitriles - toxicity ; Noctuidae ; Oligonucleotide Array Sequence Analysis ; Organophosphates ; Organophosphates - toxicity ; Oryza ; Pesticides ; Plutella xylostella ; Proteins ; Pyrethrins - toxicity ; Pyrethroids ; Real-Time Polymerase Chain Reaction ; Sequence Alignment ; Sodium ; Sodium channels (voltage-gated) ; Sodium Channels - chemistry ; Sodium Channels - metabolism ; Spodoptera - drug effects ; Spodoptera - genetics ; Spodoptera frugiperda ; Studies
É parte de: PloS one, 2013-04, Vol.8 (4), p.e62268
Notas: Conceived and designed the experiments: RAC CO MSW CB. Performed the experiments: RAC CO CB. Analyzed the data: RAC CO CB. Wrote the paper: RAC CO LMF MSW CB.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: The fall armyworm Spodoptera frugiperda is an economically important pest of small grain crops that occurs in all maize growing regions of the Americas. The intensive use of chemical pesticides for its control has led to the selection of resistant populations, however, to date, the molecular mechanisms underlying resistance have not been characterised. In this study the mechanisms involved in the resistance of two S. frugiperda strains collected in Brazil to chlorpyrifos (OP strain) or lambda-cyhalothrin (PYR strain) were investigated using molecular and genomic approaches. To examine the possible role of target-site insensitivity the genes encoding the organophosphate (acetylcholinesterase, AChE) and pyrethroid (voltage-gated sodium channel, VGSC) target-site proteins were PCR amplified. Sequencing of the S. frugiperda ace-1 gene identified several nucleotide changes in the OP strain when compared to a susceptible reference strain (SUS). These result in three amino acid substitutions, A201S, G227A and F290V, that have all been shown previously to confer organophosphate resistance in several other insect species. Sequencing of the gene encoding the VGSC in the PYR strain, identified mutations that result in three amino acid substitutions, T929I, L932F and L1014F, all of which have been shown previously to confer knockdown/super knockdown-type resistance in several arthropod species. To investigate the possible role of metabolic detoxification in the resistant phenotype of the OP and PYR stains all EST sequences available for S. frugiperda were used to design a gene-expression microarray. This was then used to compare gene expression in the resistant strains with the susceptible reference strain. Members of several gene families, previously implicated in metabolic resistance in other insects were found to be overexpressed in the resistant strains including glutathione S-transferases, cytochrome P450s and carboxylesterases. Taken together these results provide evidence that both target-site and metabolic mechanisms underlie the resistance of S. frugiperda to pyrethroids and organophosphates.
Editor: United States: Public Library of Science
Idioma: Inglês

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Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda

Carvalho, Renato A ; Omoto, Celso ; Field, Linda M ; Williamson, Martin S ; Bass, Chris Vontas, John

United States: Public Library of Science

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