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EFFECT OF BIOACTIVE ALDEHYDES ON GELATIN PROPERTIES

Krysyuk, I P ; Dzvonkevych, N D ; Volodina, T T ; Popova, N N ; Shandrenko, S G

Ukrainian biochemical journal, 2015-03, Vol.87 (2), p.113-121 [Periódico revisado por pares]

Ukraine: National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry

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Título:
EFFECT OF BIOACTIVE ALDEHYDES ON GELATIN PROPERTIES
Autor: Krysyuk, I P ; Dzvonkevych, N D ; Volodina, T T ; Popova, N N ; Shandrenko, S G
Assuntos: Acrolein - chemistry ; adducts ; aldehydes ; Biomimetic Materials ; Buffers ; Collagen - chemistry ; Cross-Linking Reagents - chemistry ; Deoxyribose - chemistry ; Fluorescence ; Formaldehyde - chemistry ; gelatin ; Gelatin - chemistry ; Glyoxal - chemistry ; Hydrogen-Ion Concentration ; protein modification ; Pyruvaldehyde - chemistry ; Ribose - chemistry ; Solutions ; Spectrometry, Fluorescence
É parte de: Ukrainian biochemical journal, 2015-03, Vol.87 (2), p.113-121
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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Descrição: Bioactive aldehydes are among main factors of proteins postsynthetic modifications, which are the cause and consequence of many diseases. Comparative study of some aldehydes modifying action on gelatin was carried out in vitro. Gelatin samples (20 mM) were incubated with: ribose, deoxyribose, glyoxal, methylglyoxal, formaldehyde, acrolein (20 mM each) and their combinations in 0.1 M Naphosphate buffer (pH 7.4) containing 0.02% sodium azide at 37 °C in the dark for 30 days. We investigated the fluorescent properties of these samples and their molecular weight distribution by electrophoresis. It has been revealed that formed adducts had different fluorescence spectra. According to fluorescence intensity these aldehydes were put in order: formaldehyde < methylglyoxal < acrolein
Editor: Ukraine: National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
Idioma: Ucraniano;Inglês

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EFFECT OF BIOACTIVE ALDEHYDES ON GELATIN PROPERTIES

Krysyuk, I P ; Dzvonkevych, N D ; Volodina, T T ; Popova, N N ; Shandrenko, S G

Ukraine: National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry

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