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GhLBDs Promote Callus Initiation and Act as Selectable Markers to Increase Transformation Efficiency

Wang, Ye ; Yuan, Jiachen ; Wei, Xi ; Chen, Yanli ; Chen, Quanjia ; Ge, Xiaoyang

Frontiers in plant science, 2022-03, Vol.13, p.861706-861706 [Periódico revisado por pares]

Switzerland: Frontiers Media S.A

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  • Título:
    GhLBDs Promote Callus Initiation and Act as Selectable Markers to Increase Transformation Efficiency
  • Autor: Wang, Ye ; Yuan, Jiachen ; Wei, Xi ; Chen, Yanli ; Chen, Quanjia ; Ge, Xiaoyang
  • Assuntos: auxin ; callus ; GhLBDs ; Gossypium hirsutum L ; Plant Science ; transformation efficiency
  • É parte de: Frontiers in plant science, 2022-03, Vol.13, p.861706-861706
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    This article was submitted to Plant Cell Biology, a section of the journal Frontiers in Plant Science
    Reviewed by: Lu Long, Henan University, China; Yijie Fan, Shandong Agricultural University, China
    These authors have contributed equally to this work
    Edited by: Fanchang Zeng, Shandong Agricultural University, China
  • Descrição: Detached organs or differentiated tissues could form a mass of pluripotent cells termed as callus on an auxin-rich medium, the underlying molecular mechanism of which remains elusive in cotton. LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factor is a key regulator of plant cell totipotency/pluripotency, and a number of cotton with high-level differential expression during the callus induction process have been identified. Their overexpression in cotton calli fostered promotions in and callus induction without exogenous auxin. Expression analysis and histological observation using paraffin sectioning suggested that the first 72 h on culture is a key time point for callus initiation, whereby the showed high transcript abundance and enlarged calli that were rapidly developed from procambium and cambium. expression level could be precisely modulated by the gradient concentrations of exogenous auxin, whereas auxin transport inhibitor 2,3,5-triiodobenzoic acid could severely inhibit its expression. The LBD-mediated callus formation was also dependent on the expression levels of . Further, a β-estradiol-inducible promoter was used to drive expression, which led to rapid callus proliferation, suggesting that / could be used as a selectable marker system to replace the existing antibiotic/herbicide-resistance selectable markers in plant transformation. Our study provides new insights for callus initiation regulatory mechanism and strategies for improving transformation efficiency in cotton.
  • Editor: Switzerland: Frontiers Media S.A
  • Idioma: Inglês
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