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Development and validation of a rapid LC-MS/MS method for the determination of JCC76, a novel antitumor agent for breast cancer, in rat plasma and its application to a pharmacokinetics study

Cai, Xiaohan ; Zhong, Bo ; Su, Bin ; Xu, Songlin ; Guo, Baochuan

Biomedical chromatography, 2012-09, Vol.26 (9), p.1118-1124 [Periódico revisado por pares]

Chichester, UK: John Wiley & Sons, Ltd

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Título:
Development and validation of a rapid LC-MS/MS method for the determination of JCC76, a novel antitumor agent for breast cancer, in rat plasma and its application to a pharmacokinetics study
Autor: Cai, Xiaohan ; Zhong, Bo ; Su, Bin ; Xu, Songlin ; Guo, Baochuan
Assuntos: Animals ; Antineoplastic Agents - blood ; Antineoplastic Agents - chemistry ; Antineoplastic Agents - pharmacokinetics ; breast cancer ; Chromatography, High Pressure Liquid - methods ; Cyclohexanecarboxylic Acids - blood ; Cyclohexanecarboxylic Acids - chemistry ; Cyclohexanecarboxylic Acids - pharmacokinetics ; Drug Stability ; JCC76 ; LC-MS/MS ; Linear Models ; Liquid-Liquid Extraction ; Male ; pharmacokinetics ; rat plasma ; Rats ; Rats, Sprague-Dawley ; Reproducibility of Results ; Sulfonamides - blood ; Sulfonamides - chemistry ; Sulfonamides - pharmacokinetics ; Tandem Mass Spectrometry - methods ; Temperature
É parte de: Biomedical chromatography, 2012-09, Vol.26 (9), p.1118-1124
Notas: ark:/67375/WNG-4QZC8KX4-M
istex:7093FD5F76ED0D32CB1CDBB117F209CAA454A0C5
ArticleID:BMC1757
Descrição: ABSTRACT JCC76 is a novel nimesulide analog that selectively inhibits the human epidermal growth factor receptor 2 (HER2) overexpressing breast cancer cell proliferation and tumor progression. To support further pharmacological and toxicological studies of JCC76, a novel and rapid method using liquid chromatography and electrospray ionization tandem mass spectrometry (LC‐ESI‐MS/MS) has been developed and validated for the quantification of the compound in rat plasma. A C18 column was used for chromatographic separation, and the mobile phase was aqueous ammonium formate (pH 3.7; 5 mm)–methanol (1:9, v/v) with an isocratic elution. With a simple liquid–liquid extraction procedure using the mixture of methyl tert‐butyl ether–hexane (1:2, v/v), the mean extraction efficiency of JCC76 in rat plasma was determined as 89.5–97.3% and no obvious matrix effect was observed. This method demonstrated a linear calibration range from 0.3 to 100 ng/mL for JCC76 in rat plasma and a small volume of sample consumption. The intra‐ and inter‐assay accuracy and precision were within ±10%. The pharmacokinetics of JCC76 was also profiled using this validated method in rats. In conclusion, this rapid and sensitive method has been proven to effectively quantify JCC76 for pharmacokinetics study. Copyright © 2011 John Wiley & Sons, Ltd.
Editor: Chichester, UK: John Wiley & Sons, Ltd
Idioma: Inglês

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Development and validation of a rapid LC-MS/MS method for the determination of JCC76, a novel antitumor agent for breast cancer, in rat plasma and its application to a pharmacokinetics study

Cai, Xiaohan ; Zhong, Bo ; Su, Bin ; Xu, Songlin ; Guo, Baochuan

Chichester, UK: John Wiley & Sons, Ltd

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