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Dynamics of clonal and plasmid backgrounds of Enterobacteriaceae producing acquired AmpC in Portuguese clinical settings over time

Ribeiro, T.G. ; Novais, Â. ; Rodrigues, C. ; Nascimento, R. ; Freitas, F. ; Machado, E. ; Peixe, L.

International journal of antimicrobial agents, 2019-05, Vol.53 (5), p.650-656 [Periódico revisado por pares]

Netherlands: Elsevier B.V

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  • Título:
    Dynamics of clonal and plasmid backgrounds of Enterobacteriaceae producing acquired AmpC in Portuguese clinical settings over time
  • Autor: Ribeiro, T.G. ; Novais, Â. ; Rodrigues, C. ; Nascimento, R. ; Freitas, F. ; Machado, E. ; Peixe, L.
  • Assuntos: Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; beta-Lactamases - biosynthesis ; beta-Lactamases - genetics ; Capsular type KL105 ; CMY-2 ; DHA-1 ; E. coli ; Enterobacteriaceae Infections - epidemiology ; Enterobacteriaceae Infections - genetics ; Enterobacteriaceae Infections - microbiology ; Humans ; IncI1 ; IncR ; K. pneumoniae ST11 ; Microbial Sensitivity Tests ; Molecular Typing ; Plasmids - analysis ; Portugal - epidemiology ; Prevalence ; Whole Genome Sequencing
  • É parte de: International journal of antimicrobial agents, 2019-05, Vol.53 (5), p.650-656
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: •Higher frequency of DHA-1 among qAmpC-producing Enterobacteriaceae.•Persistence of DHA-1 associated with IncR in ST11 K. pneumoniae with KL105.•Recent increase of blaCMY-2 associated with IncI1plasmids among polyclonal E. coli in hospitalized patients.•Different genetic backgrounds contributing to ESBL/qAmpC spread in our clinical settings. The objective of this work was to provide detailed molecular data on clinically acquired AmpC (qAmpC)-producing Enterobacteriaceae from two different periods (2002–2008 and 2010–2013) in order to clarify the contribution of clonal and plasmid genetic platforms for the current epidemiological scenario concerning extended-spectrum beta-lactams resistance. We analysed 1246 Enterobacteriaceae non-susceptible to third-generation cephalosporins from two hospitals and one community laboratory between 2010 and 2013. Bacterial identification, antibiotic susceptibility, identification of qAmpC and plasmid-mediated quinolone resistance genes, clonal (pulsed-field gel electrophoresis (PFGE), Multilocus sequence typing (MLST)) and plasmid (S1-/I-CeuI-PFGE, replicon typing, hybridization) analysis were performed by standard methods. Whole-genome sequencing (WGS) was performed in two ST11-Klebsiella pneumoniae isolates harbouring DHA-1. The occurrence of qAmpC was lower (2.6%) than that observed in a previous survey (7.4%), and varied slightly over time. Isolates produced DHA-1 (53%), CMY-2 (44%) or DHA-6 (3%), but significant epidemiological changes were observed in the two surveys. While DHA-1 persisted in different institutions by selection of a worldwide epidemic IncR plasmid in an ST11 harbouring KL105, CMY-2 rates increased over time linked to IncI1 plasmids (instead of IncK or IncA/C2) in multiple Escherichia coli clones. The higher frequency of DHA-1 qAmpC in these species contrasts with the scenario in most European countries. Furthermore, the different genetic backgrounds associated with either extended-spectrum β-lactamases (ESBLs) or acquired AmpC β-lactamases (qAmpC) in our country might have contributed to their differential expansion.
  • Editor: Netherlands: Elsevier B.V
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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