skip to main content
Idioma:
Primo Search
Clear Search Box
Search in: Busca Geral
Busca Avançada
Busca por Índices

Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study

DE MORAES, Maria Denise Rodrigues ; PASSOS, Vanara Florêncio ; PADOVANI, Gislaine Cristina ; BEZERRA, Lady Clarissa Brito da Rocha ; VASCONCELOS, Ilka Maria ; SANTIAGO, Sérgio Lima

Brazilian oral research, 2021-01, Vol.35, p.e108-e108 [Periódico revisado por pares]

Sociedade Brasileira de Pesquisa Odontológica - SBPqO

Texto completo disponível

Citações Citado por
  • Título:
    Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study
  • Autor: DE MORAES, Maria Denise Rodrigues ; PASSOS, Vanara Florêncio ; PADOVANI, Gislaine Cristina ; BEZERRA, Lady Clarissa Brito da Rocha ; VASCONCELOS, Ilka Maria ; SANTIAGO, Sérgio Lima
  • Assuntos: Camellia sinensis ; Collagen ; Dentistry ; DENTISTRY, ORAL SURGERY & MEDICINE ; Matrix Metalloproteinases ; Tooth Erosion ; Tooth Wear
  • É parte de: Brazilian oral research, 2021-01, Vol.35, p.e108-e108
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
  • Descrição: Abstract The present study sought to evaluate the protective effect of Epigallocatechin-3-gallate (EGCG) and commercial green tea (GT) on eroded dentin using in vitro and in situ experimental models. For the in vitro experiment, matrix metalloproteinases (MMPs) were extracted from demineralized human coronary dentin powder (citric acid, pH 2.3) and assessed via a colorimetric assay and electrophoresis in gelatin. The gels were exposed to buffers with: control (no treatment), 0.05% sodium fluoride (NaF), 0.12% chlorhexidine digluconate (CHX), GT infusion, and 0.1% EGCG, and their respective activity was analyzed by zymography. For the in situ experiment, 20 healthy volunteers (aged 20-32 years) participated in this single-center, blind, crossover study. The subjects wore upper removable devices containing four human dentin blocks. Erosive challenge (coke-1 min) was performed four times/day/5 days. Blocks were treated for 1 min with: control (No treatment), 0.05% NaF, 0.1% EGCG, and GT. Thereafter, the specimens were subjected to stylus profilometry and SEM. ANOVA was used to evaluate dentin roughness and wear, with a significance level of 5%. In the zymography analysis, 0.12% CHX, GT, and 0.1% EGCG were found to inhibit the action of MMPs; however, in the colorimetric assay, only green tea inhibited the activity of MMPs. There were no significant differences observed in dentin roughness or wear (p > 0.05). Herein, EGCG and GT inhibited the activity of endogenous proteases, resulting in protection against erosion-induced dentin damage; however, they could not prevent tooth tissue loss in situ.
  • Editor: Sociedade Brasileira de Pesquisa Odontológica - SBPqO
  • Idioma: Inglês;Português
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.