Idioma:

Rescue of Escherichia coli cells from UV-induced death and filamentation by caspase-3 inhibitor

Wadhawan, Surbhi ; Gautam, Satyendra

International microbiology, 2019-09, Vol.22 (3), p.369-376 [Periódico revisado por pares]

Cham: Springer International Publishing

Texto completo disponível

Citações Citado por

Enviar para

Título:
Rescue of Escherichia coli cells from UV-induced death and filamentation by caspase-3 inhibitor
Autor: Wadhawan, Surbhi ; Gautam, Satyendra
Assuntos: Apoptosis ; Applied Microbiology ; Biomedical and Life Sciences ; Caspase 3 - metabolism ; Caspase Inhibitors - metabolism ; Caspase-3 ; Cell death ; Cell survival ; Cysteine Proteinase Inhibitors - metabolism ; E coli ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - enzymology ; Escherichia coli - growth & development ; Escherichia coli - radiation effects ; Eukaryotic Microbiology ; Exposure ; Filamentation ; Filaments ; Fluorescent dyes ; Fluorescent indicators ; Fluorides ; Gamma rays ; Irradiation ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbial Viability - drug effects ; Microbial Viability - radiation effects ; Microbiology ; Original Article ; Protease ; Protease inhibitors ; Proteinase inhibitors ; Radiation ; Serine proteinase ; Serine Proteinase Inhibitors - metabolism ; SOS response ; SOS Response, Genetics - drug effects ; SOS Response, Genetics - radiation effects ; Survival ; Ultraviolet radiation ; Ultraviolet Rays
É parte de: International microbiology, 2019-09, Vol.22 (3), p.369-376
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Escherichia coli cells have been observed earlier to display caspase-3-like protease activity (CLP) and undergo programmed cell death (PCD) when exposed to gamma rays. The presence of an irreversible caspase-3 inhibitor (Ac-DEVD-CMK) during irradiation was observed to increase cell survival. Since radiation is known to induce SOS response, the effect of a caspase-3 inhibitor on SOS response was studied in E. coli . UV, a well-known SOS inducer, was used in the current study. Cell filamentation in E. coli upon UV exposure was found to be inhibited by ninefold in the presence of a caspase-3 inhibitor. CLP activity was found to increase twofold in UV-exposed cells than in control (non-treated) cells. Further, bright fluorescing filaments were observed in UV-exposed E. coli cells treated with FITC-DEVD-FMK, a fluorescent dye tagged with an irreversible caspase-3 inhibitor (DEVD-FMK), indicating the presence of active CLP in these cells. Unlike caspase-3 inhibitor, a serine protease inhibitor, phenylmethanesulfonyl fluoride (PMSF), was not found to improve cell survival after UV treatment. Additionally, a SOS reporter system known as SIVET (selectable in vivo expression technology) assay was performed to reconfirm the inhibition of SOS induction in the presence of caspase-3 inhibitor. SIVET assay is used to quantify cells in which the SOS response has been induced leading to a scorable permanent selectable change in the cell. The SIVET induction frequency (calculated as the ratio of SIVET-induced cells to total viable cells) increased around tenfold in UV-exposed cultures. The induction frequency was found to decrease significantly to 51 from 80% in the cells pre-incubated with caspase-3 inhibitor. On the contrary, caspase-3 inhibitor failed to improve cell survival of E. coli Δ recA and E. coli DM49 (SOS non-inducible) cells post UV treatment. Summing together, the results indicated a possible linkage of SOS response and the PCD process in E. coli . The findings also indicated that functional SOS pathway is required for CLP-like activity; however, the exact mechanism remains to be elucidated.
Editor: Cham: Springer International Publishing
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Rescue of Escherichia coli cells from UV-induced death and filamentation by caspase-3 inhibitor

Wadhawan, Surbhi ; Gautam, Satyendra

Cham: Springer International Publishing

Buscando em bases de dados remotas. Favor aguardar.