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A flavin-based extracellular electron transfer mechanism in diverse Gram-positive bacteria

Light, Samuel H ; Su, Lin ; Rivera-Lugo, Rafael ; Cornejo, Jose A ; Louie, Alexander ; Iavarone, Anthony T ; Ajo-Franklin, Caroline M ; Portnoy, Daniel A

Nature (London), 2018-10, Vol.562 (7725), p.140-144 [Periódico revisado por pares]

England: Nature Publishing Group

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Título:
A flavin-based extracellular electron transfer mechanism in diverse Gram-positive bacteria
Autor: Light, Samuel H ; Su, Lin ; Rivera-Lugo, Rafael ; Cornejo, Jose A ; Louie, Alexander ; Iavarone, Anthony T ; Ajo-Franklin, Caroline M ; Portnoy, Daniel A
Assuntos: Aerobic respiration ; Aerobiosis ; Animals ; Bacteria ; Benzoquinones - metabolism ; Carbon sources ; Cell activation ; Cell Respiration ; Channeling ; Communicable diseases ; Cytosol ; Dehydrogenases ; Electrodes ; Electron transfer ; Electron transport ; Electron Transport - genetics ; Electrons ; Enzymes ; Female ; Firmicutes - enzymology ; Firmicutes - genetics ; Firmicutes - metabolism ; Flavin ; Flavins - metabolism ; Gastrointestinal system ; Gastrointestinal tract ; Gastrointestinal Tract - microbiology ; Gene mutation ; Gene mutations ; Genes ; Genetic screening ; Genetic testing ; Gram-positive bacteria ; Gram-Positive Bacteria - enzymology ; Gram-Positive Bacteria - genetics ; Gram-Positive Bacteria - metabolism ; Haem ; Heme ; Infections ; Infectious diseases ; Intestinal microflora ; Intestine ; Iron ; Iron - chemistry ; Iron compounds ; Listeria ; Listeria monocytogenes ; Listeria monocytogenes - enzymology ; Listeria monocytogenes - genetics ; Listeria monocytogenes - metabolism ; Loci ; Membrane structure ; Membrane structures ; Mice ; Microbial activity ; Microbiota ; Microbiota (Symbiotic organisms) ; Microorganisms ; Mutants ; NADH ; NADH dehydrogenase ; NADH Dehydrogenase - metabolism ; Nicotinamide adenine dinucleotide ; Pathogenic microorganisms ; Pathogens ; Proteins ; Proteomics ; Quinones ; Reductase
É parte de: Nature (London), 2018-10, Vol.562 (7725), p.140-144
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
AC02-05CH11231
USDOE Office of Science (SC), Basic Energy Sciences (BES)
S.H.L., A.T.I., C.M.A-F., and D.A.P. designed the study. S.H.L, L.S., and J.A.C. performed electrochemical experiments. S.H.L. and A.T.I. performed mass spectrometric experiments. S.H.L., A.L., and R.R-L. performed microbiological and biochemical experiments. S.H.L. and D.A.P. wrote the manuscript.
D.A.P. has a consulting relationship with and a financial interest in Aduro Biotech; both he and the company stand to benefit from the commercialization of this research. Correspondence and requests for materials should be addressed to D.A.P. (portnoy@berkeley.edu).
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Descrição: Extracellular electron transfer (EET) describes microbial bioelectrochemical processes in which electrons are transferred from the cytosol to the exterior of the cell . Mineral-respiring bacteria use elaborate haem-based electron transfer mechanisms but the existence and mechanistic basis of other EETs remain largely unknown. Here we show that the food-borne pathogen Listeria monocytogenes uses a distinctive flavin-based EET mechanism to deliver electrons to iron or an electrode. By performing a forward genetic screen to identify L. monocytogenes mutants with diminished extracellular ferric iron reductase activity, we identified an eight-gene locus that is responsible for EET. This locus encodes a specialized NADH dehydrogenase that segregates EET from aerobic respiration by channelling electrons to a discrete membrane-localized quinone pool. Other proteins facilitate the assembly of an abundant extracellular flavoprotein that, in conjunction with free-molecule flavin shuttles, mediates electron transfer to extracellular acceptors. This system thus establishes a simple electron conduit that is compatible with the single-membrane structure of the Gram-positive cell. Activation of EET supports growth on non-fermentable carbon sources, and an EET mutant exhibited a competitive defect within the mouse gastrointestinal tract. Orthologues of the genes responsible for EET are present in hundreds of species across the Firmicutes phylum, including multiple pathogens and commensal members of the intestinal microbiota, and correlate with EET activity in assayed strains. These findings suggest a greater prevalence of EET-based growth capabilities and establish a previously underappreciated relevance for electrogenic bacteria across diverse environments, including host-associated microbial communities and infectious disease.
Editor: England: Nature Publishing Group
Idioma: Inglês

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A flavin-based extracellular electron transfer mechanism in diverse Gram-positive bacteria

Light, Samuel H ; Su, Lin ; Rivera-Lugo, Rafael ; Cornejo, Jose A ; Louie, Alexander ; Iavarone, Anthony T ; Ajo-Franklin, Caroline M ; Portnoy, Daniel A

England: Nature Publishing Group

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