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Urinary cell-free DNA is a versatile analyte for monitoring infections of the urinary tract

Burnham, Philip ; Dadhania, Darshana ; Heyang, Michael ; Chen, Fanny ; Westblade, Lars F ; Suthanthiran, Manikkam ; Lee, John Richard ; De Vlaminck, Iwijn

Nature communications, 2018-06, Vol.9 (1), p.2412-10, Article 2412 [Periódico revisado por pares]

England: Nature Publishing Group

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Urinary cell-free DNA is a versatile analyte for monitoring infections of the urinary tract
Autor: Burnham, Philip ; Dadhania, Darshana ; Heyang, Michael ; Chen, Fanny ; Westblade, Lars F ; Suthanthiran, Manikkam ; Lee, John Richard ; De Vlaminck, Iwijn
Assuntos: Bacteria ; Biomarkers - urine ; Cell culture ; Cell-Free Nucleic Acids - isolation & purification ; Cell-Free Nucleic Acids - urine ; Cohort Studies ; Deoxyribonucleic acid ; Diagnostic systems ; DNA ; DNA, Bacterial - isolation & purification ; DNA, Bacterial - urine ; DNA, Viral - isolation & purification ; DNA, Viral - urine ; Female ; High-Throughput Nucleotide Sequencing ; Host-Pathogen Interactions ; Humans ; Infections ; Kidney transplantation ; Kidneys ; Male ; Microbiomes ; Microbiota - genetics ; Sequence Analysis, DNA ; Syngeneic grafts ; Urinalysis ; Urinary tract ; Urinary Tract - microbiology ; Urinary tract diseases ; Urinary tract infections ; Urinary Tract Infections - diagnosis ; Urinary Tract Infections - microbiology ; Urinary Tract Infections - urine ; Urine ; Urogenital system
É parte de: Nature communications, 2018-06, Vol.9 (1), p.2412-10, Article 2412
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SourceType-Scholarly Journals-1
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Descrição: Urinary tract infections are one of the most common infections in humans. Here we tested the utility of urinary cell-free DNA (cfDNA) to comprehensively monitor host and pathogen dynamics in bacterial and viral urinary tract infections. We isolated cfDNA from 141 urine samples from a cohort of 82 kidney transplant recipients and performed next-generation sequencing. We found that urinary cfDNA is highly informative about bacterial and viral composition of the microbiome, antimicrobial susceptibility, bacterial growth dynamics, kidney allograft injury, and host response to infection. These different layers of information are accessible from a single assay and individually agree with corresponding clinical tests based on quantitative PCR, conventional bacterial culture, and urinalysis. In addition, cfDNA reveals the frequent occurrence of pathologies that remain undiagnosed with conventional diagnostic protocols. Our work identifies urinary cfDNA as a highly versatile analyte to monitor infections of the urinary tract.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Urinary cell-free DNA is a versatile analyte for monitoring infections of the urinary tract

Burnham, Philip ; Dadhania, Darshana ; Heyang, Michael ; Chen, Fanny ; Westblade, Lars F ; Suthanthiran, Manikkam ; Lee, John Richard ; De Vlaminck, Iwijn

England: Nature Publishing Group

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