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Aptamer Laden Liquid Crystals Biosensing Platform for the Detection of HIV-1 Glycoprotein-120

Abbasi, Amna Didar ; Hussain, Zakir ; Yang, Kun-Lin

Molecules (Basel, Switzerland), 2021-05, Vol.26 (10), p.2893 [Periódico revisado por pares]

Basel: MDPI AG

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Título:
Aptamer Laden Liquid Crystals Biosensing Platform for the Detection of HIV-1 Glycoprotein-120
Autor: Abbasi, Amna Didar ; Hussain, Zakir ; Yang, Kun-Lin
Assuntos: Aptamers ; B40t77 ; biosensor ; Biosensors ; Bovine serum albumin ; Capsid protein ; Chloride ; Crystals ; DMOAP ; Glycoproteins ; gp-120 ; Hepatitis ; Hepatitis A ; HIV ; Human immunodeficiency virus ; IgG antibody ; Image analysis ; Image processing ; Immunoglobulin G ; Immunoglobulins ; Liquid crystals ; Medical research ; Nitrogen ; Optical microscopes ; Proteins ; RNA aptamer ; Selectivity ; Serum albumin ; VP1 protein
É parte de: Molecules (Basel, Switzerland), 2021-05, Vol.26 (10), p.2893
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: We report a label-free and simple approach for the detection of glycoprotein-120 (gp-120) using an aptamer-based liquid crystals (LCs) biosensing platform. The LCs are supported on the surface of a modified glass slide with a suitable amount of B40t77 aptamer, allowing the LCs to be homeotropically aligned. A pronounced topological change was observed on the surface due to a specific interaction between B40t77 and gp-120, which led to the disruption of the homeotropic alignment of LCs. This results in a dark-to-bright transition observed under a polarized optical microscope. With the developed biosensing platform, it was possible to not only identify gp-120, but obtained results were analyzed quantitatively through image analysis. The detection limit of the proposed biosensing platform was investigated to be 0.2 μg/mL of gp-120. Regarding selectivity of the developed platform, no response could be detected when gp-120 was replaced by other proteins, such as bovine serum albumin (BSA), hepatitis A virus capsid protein 1 (Hep A VP1) and immunoglobulin G protein (IgG). Due to attributes such as label-free, high specificity and no need for instrumental read-out, the presented biosensing platform provides the potential to develop a working device for the quick detection of HIV-1 gp-120.
Editor: Basel: MDPI AG
Idioma: Inglês

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Aptamer Laden Liquid Crystals Biosensing Platform for the Detection of HIV-1 Glycoprotein-120

Abbasi, Amna Didar ; Hussain, Zakir ; Yang, Kun-Lin

Basel: MDPI AG

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