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Protein acetylation methods and protocols

Sandra B Hake; Christian J Janzen

New York Humana Press Springer c2013

Localização: CQ - Conjunto das Químicas    (574.19245 P8953 )(Acessar)

  • Título:
    Protein acetylation methods and protocols
  • Autor: Sandra B Hake; Christian J Janzen
  • Assuntos: Acetylation -- Laboratory Manuals; Proteins -- Laboratory Manuals; Acetylation -- Laboratory manuals; Proteins -- Laboratory manuals; PROTEÍNAS; REAÇÕES QUÍMICAS
  • Notas: Includes bibliographical references and index ; Includes bibliogaphical references and index
  • Descrição: Validation of protein acetylation by mass spectrometry Application of the CIRAD mass spectrometry approach for lysine acetylation site discovery Application of the MIDAS approach for analysis of lysine acetylation sites Application of high content biology to yield quantitative spatial proteomic information on protein acetylations Towards the N-Terminal acetylome : an N-Terminal acetylated peptide enrichment method using CNBr-activated sepharose resin Identification and analysis of o-acetylated sialoglycoproteins HPLC-based quantification of in vitro N-terminal acetylation Separation and purification of multiply acetylated proteins using cation-exchange chromatography In-gel N-acetylation for the quantification of the degree of protein in vivo N-terminal acetylation Computational prediction of lysine acetylation proteome-wide Generation and characterization of pan-specific anti-acetyllysine antibody Using functional proteome microarrays to study protein lysine acetylation Quantitation of nucleosome acetylation and other histone posttranslational modifications using microscale NU-ELISA Preparing semisynthetic and fully synthetic histones H3 and H4 to modify the nucleosome core Production of amino-terminally acetylated recombinant proteins in E. coli / Identification of lysine acetyltransferase substrates using bioorthogonal chemical proteomics
    Nonradioactive in vitro assays for histone deacetylases Fluorescence-based acetylation assay using thiol-sensitive probes Analysis of protein acetyltransferase structure-function relation by surface-enhanced raman scattering (SERS): a tool to screen and characterize small molecule modulators
  • Títulos relacionados: Série:Methods in molecular biology (Clifton, N.J.) v. 981 1064-3745; Springer protocols (Series)
  • Editor: New York Humana Press Springer
  • Data de criação/publicação: c2013
  • Formato: xi, 266 p ill. (some col.) cm..
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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