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Detection of Brazilian Hantavirus by Reverse Transcription Polymerase Chain Reaction Amplification of N Gene in Patients with Hantavirus Cardiopulmonary Syndrome

Moreli, Marcos Lázaro ; Sousa, Ricardo Luiz Moro de ; Figueiredo, Luiz Tadeu Moraes

Memórias do Instituto Oswaldo Cruz, 2004-10, Vol.99 (6), p.633-638 [Periódico revisado por pares]

Brazil: Fundação Oswaldo Cruz, Fiocruz

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Título:
Detection of Brazilian Hantavirus by Reverse Transcription Polymerase Chain Reaction Amplification of N Gene in Patients with Hantavirus Cardiopulmonary Syndrome
Autor: Moreli, Marcos Lázaro ; Sousa, Ricardo Luiz Moro de ; Figueiredo, Luiz Tadeu Moraes
Assuntos: Adolescent ; Adult ; Base Sequence ; Case-Control Studies ; Enzyme-Linked Immunosorbent Assay ; Female ; Genome, Viral ; Hantavirus ; Hantavirus - genetics ; Hantavirus - isolation & purification ; hantavirus cardiopulmonary syndrome ; Hantavirus Pulmonary Syndrome - diagnosis ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; N gene of hantavirus ; nested RT-PCR ; PARASITOLOGY ; Reverse Transcriptase Polymerase Chain Reaction - methods ; TROPICAL MEDICINE
É parte de: Memórias do Instituto Oswaldo Cruz, 2004-10, Vol.99 (6), p.633-638
Notas: ObjectType-Article-2
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Descrição: We report a nested reverse transcription-polymerase chain reaction (RT-PCR) assay for hantavirus using primers selected to match high homology regions of hantavirus genomes detected from the whole blood of hantavirus cardiopulmonary syndrome (HCPS) patients from Brazil, also including the N gene nucleotide sequence of Araraquara virus. Hantavirus genomes were detected in eight out of nine blood samples from the HCPS patients by RT-PCR (88.9% positivity) and in all 9 blood samples (100% positivity) by nested-PCR. The eight amplicons obtained by RT-PCR (P1, P3-P9), including one obtained by nested-PCR (P-2) and not obtained by RT-PCR, were sequenced and showed high homology (94.8% to 99.1%) with the N gene of Araraquara hantavirus. Although the serologic method ELISA is the most appropriate test for HCPS diagnosis, the use of nested RT-PCR for hantavirus in Brazil would contribute to the diagnosis of acute hantavirus disease detecting viral genomes in patient specimens as well as initial genomic characterization of circulating hantaviruses.
Editor: Brazil: Fundação Oswaldo Cruz, Fiocruz
Idioma: Inglês;Português

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Detection of Brazilian Hantavirus by Reverse Transcription Polymerase Chain Reaction Amplification of N Gene in Patients with Hantavirus Cardiopulmonary Syndrome

Moreli, Marcos Lázaro ; Sousa, Ricardo Luiz Moro de ; Figueiredo, Luiz Tadeu Moraes

Brazil: Fundação Oswaldo Cruz, Fiocruz

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