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Identification and Profiling of Specialized Pro-Resolving Mediators in Human Tears by Lipid Mediator Metabolomics

English, Justin T ; Norris, Paul C ; Hodges, Robin R ; Dartt, Darlene A ; Serhan, Charles N

Prostaglandins, leukotrienes and essential fatty acids, 2017-02, Vol.117, p.17-27 [Periódico revisado por pares]

Scotland: Elsevier Ltd

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Título:
Identification and Profiling of Specialized Pro-Resolving Mediators in Human Tears by Lipid Mediator Metabolomics
Autor: English, Justin T ; Norris, Paul C ; Hodges, Robin R ; Dartt, Darlene A ; Serhan, Charles N
Assuntos: Adolescent ; Adult ; Advanced Basic Science ; Child, Preschool ; Chromatography, Liquid ; Docosahexaenoic Acids - analysis ; Endocrinology & Metabolism ; Female ; Humans ; inflammation ; Leukotriene A4 - analysis ; Lipid Mediators ; Lipoxins - analysis ; Male ; Metabolomics - methods ; Middle Aged ; Omega-3 fatty acids ; Principal Component Analysis ; Prostaglandins - analysis ; protectins ; resolution ; resolvins ; Sex Characteristics ; Tandem Mass Spectrometry ; Tears - chemistry ; Young Adult
É parte de: Prostaglandins, leukotrienes and essential fatty acids, 2017-02, Vol.117, p.17-27
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Contributed equally
Descrição: Abstract Specialized pro-resolving mediators (SPM), e.g. Resolvin D1, Protectin D1, Lipoxin A₄, and Resolvin E1 have each shown to be active in ocular models reducing inflammation. In general, SPMs have specific agonist functions that stimulate resolution of infection and inflammation in animal disease models. The presence and quantity of SPM in human emotional tears is of interest. Here, utilizing a targeted LC-MS-MS metabololipidomics based approach we document the identification of pro-inflammatory (Prostaglandins and Leukotriene B₄) and pro-resolving lipid mediators (D-series Resolvins, Protectin D1, and Lipoxin A₄) in human emotional tears from 12 healthy individuals. SPMs from the Maresin family (Maresin 1 and Maresin 2) were not present in these samples. Principal Component Analysis (PCA) revealed gender differences in the production of specific mediators within these tear samples as the SPMs were essentially absent in these female donors. These results indicate that specific SPM signatures are present in human emotional tears at concentrations known to be bioactive. Moreover, they will help to further appreciate the mechanisms of production and action of SPMs in the eye, as well as their physiologic roles in human ocular disease resolution.
Editor: Scotland: Elsevier Ltd
Idioma: Inglês

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Identification and Profiling of Specialized Pro-Resolving Mediators in Human Tears by Lipid Mediator Metabolomics

English, Justin T ; Norris, Paul C ; Hodges, Robin R ; Dartt, Darlene A ; Serhan, Charles N

Scotland: Elsevier Ltd

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