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Factors that affect the embryo production and fertility of buffalo donors and recipientes

Chello, Damiana

Biblioteca Digital de Teses e Dissertações da USP; Universidade de São Paulo; Faculdade de Medicina Veterinária e Zootecnia 2020-01-30

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  • Título:
    Factors that affect the embryo production and fertility of buffalo donors and recipientes
  • Autor: Chello, Damiana
  • Orientador: Baruselli, Pietro Sampaio
  • Assuntos: Tet; Amh; Búfalo; Gnrh; Categoria; Corpo Lúteo Acessório; Tet; Category; Buffalo; Accessory Corpus Luteum
  • Notas: Dissertação (Mestrado)
  • Descrição: Currently, ultrasound-guided follicular aspiration (OPU) combined with in vitro embryo production (IVEP) are technologies that show promising results in buffalo to multiply individuals of high genetic value. The peculiar reproductive characteristics of the buffalo species have made superovulation and in vivo embryo production a low efficiency technology. To better evaluate the response to OPU/IVEP, two studies were proposed in buffalo donors and recipients. In Experiment 1, the effect of the donor category (prepubertal and pubertal heifers and cows) and donor anti-Müllerian (AMH) plasma concentrations on the efficiency of OPU/IVEP was studied. Seventy- two Murrah donors belonging to the three categories were used. The animals were aspirated on the same day and IVEP was performed with the same semen batch of a single bull. At the time of OPU a blood sample was taken to measure circulating AMH levels. The prepubertal category showed lower embryo production when compared to cows. On the other hand, adult animals (cows) presented higher viable oocyte and embryo production rates. Positive correlation was found between plasma AMH concentrations and follicle number (AFP), number of oocytes retrieved and embryo production in all categories. In Experiment 2, the effect of GnRH treatment at ET on accessory CL induction, increased plasma P4 concentrations, and embryo transfer pregnancy rate (P/ET) was evaluated. There was no effect of GnRH treatment on increasing P4 concentrations and P/TE. Low ovulation rate and accessory CL formation (only 28%) were found after GnRH treatment. However, a positive relationship was found between plasma P4 concentration and CL vascularization assessed by Color Doppler ultrasonography. It was concluded that AMH is an endocrine marker for OPU/IVEP efficiency in buffalo donors. Pre-pubertal donors have lower embryo production compared to cows. Also, treatment with GnRH at the time of ET did not increase plasma P4 concentrations and P/TE.
  • DOI: 10.11606/D.10.2020.tde-12052020-100933
  • Editor: Biblioteca Digital de Teses e Dissertações da USP; Universidade de São Paulo; Faculdade de Medicina Veterinária e Zootecnia
  • Data de criação/publicação: 2020-01-30
  • Formato: Adobe PDF
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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