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Comparative study of leishmania amazonensis in mice infected by different route

Tânia Cristina Felizardo Glória Maria Collet de Araujo Lima; Congresso Instituto Ciências Biomédicas, IV (2. 2003 São Paulo)

Resumos São Paulo: Comissão de Pesquisa do ICB/USP

São Paulo Comissão de Pesquisa do ICB/USP 2003

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  • Título:
    Comparative study of leishmania amazonensis in mice infected by different route
  • Autor: Tânia Cristina Felizardo
  • Glória Maria Collet de Araujo Lima; Congresso Instituto Ciências Biomédicas, IV (2. 2003 São Paulo)
  • Materias: IMUNOLOGIA
  • Es parte de: Resumos São Paulo: Comissão de Pesquisa do ICB/USP
  • Notas: Disponível em CD-ROM
  • Descripción: The factors that determine the severity of cutaneous leishmaniasis have been under intense investigation. We are investigating how infection at different inoculation sites of the mouse would affect lesion development and the immune response to the parasite. We chose intradermal inoculation in the footpad or in the dorsal skin at the base of the tail. Infection was performed with 10 million L.amazonensis (Josefa strain) stationary phase promastigotes in susceptible BALB/c or resistant C57BL/6 mice and in their respective IL-4 deficient (IL-4 KO) strains. Our results show that the lesions of wild type mice (WT) of either strain were more severe when infection was performed in the dorsum than in the footpad and characteristically the former ulcerated. The lesions developed by IL-4 KO mice from both strains were smaller and no lesion was observed in C57BL/6 IL-4 KO inoculated in the dorsum. A marked inflammatory infiltrate associated with amastigote nests was present in the paw and in the dorsal lesion obtained from infected WT BALB/c or WT C57BL/6 mice. In contrast, sections from IL-4 KO mice had only few, circumscribed parasite nests. The number of parasites quantified in DLN by LDA did not vary significantly between WT or IL-4 KO BALB/c or WT C57BL/6; however IL-4 KO C57BL/6 mice inoculated in the dorsum had markedly fewer parasites in the inguinal DLN in comparison to the other groups. The antibody response and isotype distribution was not
    different between mice inoculated in the dorsum or in the footpad. Serum levels of parasite-specific IgG1 and total IgE were higher in BALB/c than in C57BL/6 mice. Preliminary data suggest that IL-4 production by DLN cells is higher in animals infected in the footpad as compared to those inoculated in the dorsum. Taken together, our data indicate that the route of inoculation has a role in determining the outcome of the infection or lesion both in resistant and susceptible mice
  • Editor: São Paulo Comissão de Pesquisa do ICB/USP
  • Fecha de creación: 2003
  • Formato: Poster 82.
  • Idioma: Portugués
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