Idioma:

Genomic expression responses to DNA-damaging agents and the regulatory role of the yeast ATR homolog Mec1p

Gasch, A P ; Huang, M ; Metzner, S ; Botstein, D ; Elledge, S J ; Brown, P O Walter, Peter

Molecular biology of the cell, 2001-10, Vol.12 (10), p.2987-3003 [Periódico revisado por pares]

United States: The American Society for Cell Biology

Texto completo disponível

Citações Citado por

Enviar para

Título:
Genomic expression responses to DNA-damaging agents and the regulatory role of the yeast ATR homolog Mec1p
Autor: Gasch, A P ; Huang, M ; Metzner, S ; Botstein, D ; Elledge, S J ; Brown, P O
Walter, Peter
Assuntos: Cell Cycle Proteins - genetics ; Cell Cycle Proteins - metabolism ; DNA Damage - drug effects ; DNA Damage - radiation effects ; DNA Repair - physiology ; DNA, Fungal - metabolism ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Expression Regulation, Fungal - drug effects ; Gene Expression Regulation, Fungal - physiology ; Gene Expression Regulation, Fungal - radiation effects ; Intracellular Signaling Peptides and Proteins ; Methyl Methanesulfonate - pharmacology ; Mutation - genetics ; Oligonucleotide Array Sequence Analysis ; Protein Kinases - metabolism ; Protein Serine-Threonine Kinases ; Radiation, Ionizing ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins ; Sequence Homology ; Signal Transduction - physiology
É parte de: Molecular biology of the cell, 2001-10, Vol.12 (10), p.2987-3003
Notas: Corresponding author. E-mail address: pbrown@cmgm.stanford.edu.
These authors contributed equally to this work.
Present address: Lawrence Berkeley National Lab, Berkeley, CA 94720
Present address: Department of Biochemistry and Molecular Genetics, University of Colorado Health Science Center, Denver, CO 80262.
Descrição: Eukaryotic cells respond to DNA damage by arresting the cell cycle and modulating gene expression to ensure efficient DNA repair. The human ATR kinase and its homolog in yeast, MEC1, play central roles in transducing the damage signal. To characterize the role of the Mec1 pathway in modulating the cellular response to DNA damage, we used DNA microarrays to observe genomic expression in Saccharomyces cerevisiae responding to two different DNA-damaging agents. We compared the genome-wide expression patterns of wild-type cells and mutants defective in Mec1 signaling, including mec1, dun1, and crt1 mutants, under normal growth conditions and in response to the methylating-agent methylmethane sulfonate (MMS) and ionizing radiation. Here, we present a comparative analysis of wild-type and mutant cells responding to these DNA-damaging agents, and identify specific features of the gene expression responses that are dependent on the Mec1 pathway. Among the hundreds of genes whose expression was affected by Mec1p, one set of genes appears to represent an MEC1-dependent expression signature of DNA damage. Other aspects of the genomic responses were independent of Mec1p, and likely independent of DNA damage, suggesting the pleiotropic effects of MMS and ionizing radiation. The complete data set as well as supplemental materials is available at http://www-genome.stanford.edu/mec1.
Editor: United States: The American Society for Cell Biology
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Genomic expression responses to DNA-damaging agents and the regulatory role of the yeast ATR homolog Mec1p

Gasch, A P ; Huang, M ; Metzner, S ; Botstein, D ; Elledge, S J ; Brown, P O Walter, Peter

United States: The American Society for Cell Biology

Buscando em bases de dados remotas. Favor aguardar.