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E-cadherin is required for cranial neural crest migration in Xenopus laevis

Huang, Chaolie ; Kratzer, Marie-Claire ; Wedlich, Doris ; Kashef, Jubin

Developmental biology, 2016-03, Vol.411 (2), p.159-171 [Periódico revisado por pares]

United States: Elsevier Inc

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  • Título:
    E-cadherin is required for cranial neural crest migration in Xenopus laevis
  • Autor: Huang, Chaolie ; Kratzer, Marie-Claire ; Wedlich, Doris ; Kashef, Jubin
  • Assuntos: Animals ; Cadherin ; Cadherins - genetics ; Cadherins - physiology ; Cell Adhesion ; Cell migration ; Cell Movement ; Cell–cell adhesion ; Cytoplasm - metabolism ; Epithelial-Mesenchymal Transition ; Gene Deletion ; Gene Expression Regulation, Developmental ; Green Fluorescent Proteins - metabolism ; Immunohistochemistry ; Microscopy, Fluorescence ; Mutation ; Neural crest ; Neural Crest - embryology ; Xenopus ; Xenopus laevis ; Xenopus laevis - embryology ; Xenopus laevis - genetics ; Xenopus Proteins - genetics ; Xenopus Proteins - physiology
  • É parte de: Developmental biology, 2016-03, Vol.411 (2), p.159-171
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
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  • Descrição: The cranial neural crest (CNC) is a highly motile and multipotent embryonic cell population, which migrates directionally on defined routes throughout the embryo, contributing to facial structures including cartilage, bone and ganglia. Cadherin-mediated cell–cell adhesion is known to play a crucial role in the directional migration of CNC cells. However, migrating CNC co-express different cadherin subtypes, and their individual roles have yet to be fully explored. In previous studies, the expression of individual cadherin subtypes has been analysed using different methods with varying sensitivities, preventing the direct comparison of expression levels. Here, we provide the first comprehensive and comparative analysis of the expression of six cadherin superfamily members during different phases of CNC cell migration in Xenopus. By applying a quantitative RT-qPCR approach, we can determine the copy number and abundance of each expressed cadherin through different phases of CNC migration. Using this approach, we show for the first time expression of E-cadherin and XB/C-cadherin in CNC cells, adding them as two new members of cadherins co-expressed during CNC migration. Cadherin co-expression during CNC migration in Xenopus, in particular the constant expression of E-cadherin, contradicts the classical epithelial-mesenchymal transition (EMT) model postulating a switch in cadherin expression. Loss-of-function experiments further show that E-cadherin is required for proper CNC cell migration in vivo and also for cell protrusion formation in vitro. Knockdown of E-cadherin is not rescued by co-injection of other classical cadherins, pointing to a specific function of E-cadherin in mediating CNC cell migration. Finally, through reconstitution experiments with different E-cadherin deletion mutants in E-cadherin morphant embryos, we demonstrate that the extracellular domain, but not the cytoplasmic domain, of E-cadherin is sufficient to rescue CNC cell migration in vivo. •A first comparative analysis on the expression of six cadherins during CNC migration.•E-cadherin is expressed during CNC migration.•E-cadherin is required for CNC migration in vivo.•E-cadherin is required for cell protrusion formation in vitro.•The extracellular domain of E-cadherin is sufficient to rescue CNC migration.
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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