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Bone resorption in 'P55 IND.-/-' knockout animals is mediated by an independent RANK-RANKL pathway

I F Saconato S Fukada; T A Silva; G P Garlet; Mario Julio Avila-Campos; F Q Cunha; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)

Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006

São Paulo 2006

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  • Título:
    Bone resorption in 'P55 IND.-/-' knockout animals is mediated by an independent RANK-RANKL pathway
  • Autor: I F Saconato
  • S Fukada; T A Silva; G P Garlet; Mario Julio Avila-Campos; F Q Cunha; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)
  • Assuntos: MICROBIOLOGIA
  • É parte de: Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006
  • Notas: Disponível em CD-ROM
  • Descrição: Introduction and objectives: Periapicopaties are dental periapical inflammatory process most frequently among maxilar bone alterations. The pathogenic process can be initiated by any injury eliciting an acute immune inflammatory host response in the periapical region. However, mechanisms involved in the periapical osteolytic lesion remained unknown. Thus, this work aimed to investigate the consequence of tumor necrosis factor-alpha p55 (TNF-alpha) deletion on periapical lesion progression and determine the relationship between lesion size and osteoclastogenic factors expression.Methods and results: Wild-type (C57/BL6) and p55 knockout (p55-/-) were placed in a retraction board and had the right first molar dental pulp exposed by a quarter round bur. The exposed pulp was inoculated by a mixture of four bacterial strains: Porphyromona Gingivalis, Prevotella Nigrescens, Actynomyces viscosus and Fusobacterium nucleatun. The left molar without exposing pulp has been used as control. After 21 days, periapical lesion was evaluated by histomorphometry and the osteoclastogenic factors by real time PCR and ELISA. The histological section showed that the exposure pulp drive to bone resorption and an intense inflammatory cells infiltration while the contralateral periodontal ligament remained normal. Apical lesion areas, measured 21 days after exposition, were similar in WT (0.13 ± 0.03mm2) and p55-/- (0.17 ± 0.01mm2) mice. When comparing WT and
    p55-/- mediators we observed increased levels of IL-1 (7.79 ± 0.9 and 16.15 ± 1.0, respectively), a resorptive cytokine, and lower levels of IFN- (11.06 ± 4.4 and 2.25 ± 1.9, respectively). p55-/- animals also showed decreased levels of SDF-1 (stromal cell derived factor-1) (2 fold), MIP-1 (macrophage inflammatory protein-1 ) (3.5 fold), RANKL (receptor activator of NF ligand) (2.5 fold) and catepsin K (2 fold) while the expression of OPG (osteoprotegerin) was not significantly different. Conclusion: Although, the area of lesion was the same in WT and p55-/- animals we observed a reduction of osteoclastogenic factors expression, suggesting that the RANK/RANKL/OPG system, activated by TNF- , is not the only pathway of osteoclasts activation.
  • Editor: São Paulo
  • Data de criação/publicação: 2006
  • Formato: res. IR.058.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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