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Immunogenicity and Serological Cross-Reactivity of Saliva Proteins among Different Tsetse Species

Zhao, Xin ; Alves e Silva, Thiago Luiz ; Cronin, Laura ; Savage, Amy F ; O'Neill, Michelle ; Nerima, Barbara ; Okedi, Loyce M ; Aksoy, Serap Solano, Philippe

PLoS neglected tropical diseases, 2015-08, Vol.9 (8), p.e0004038-e0004038 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Immunogenicity and Serological Cross-Reactivity of Saliva Proteins among Different Tsetse Species
Autor: Zhao, Xin ; Alves e Silva, Thiago Luiz ; Cronin, Laura ; Savage, Amy F ; O'Neill, Michelle ; Nerima, Barbara ; Okedi, Loyce M ; Aksoy, Serap
Solano, Philippe
Assuntos: Adenosine ; Amino Acid Sequence ; Animals ; Biomarkers ; Cattle ; Cross Reactions ; Disease transmission ; Epidemics ; Experiments ; Funding ; Genetic aspects ; Glossina fuscipes ; Glossina fuscipes fuscipes ; Glossina morsitans ; Glossina morsitans morsitans ; Glossina pallidipes ; Glossina palpalis gambiensis ; Growth factors ; Health aspects ; Immunoglobulins ; Insect Proteins - chemistry ; Insect Proteins - immunology ; Insect Vectors - chemistry ; Insect Vectors - classification ; Insect Vectors - immunology ; Insect Vectors - physiology ; Male ; Mass Spectrometry ; Mice ; Mice, Inbred C57BL ; Molecular Sequence Data ; Observations ; Phylogeny ; Properties ; Proteins ; Saliva ; Salivary glands ; Salivary Proteins and Peptides - chemistry ; Salivary Proteins and Peptides - immunology ; secretions ; Surveillance ; Trypanosoma ; Trypanosoma brucei gambiense - immunology ; Trypanosoma brucei gambiense - physiology ; Trypanosomiasis, Bovine - immunology ; Trypanosomiasis, Bovine - parasitology ; Tsetse Flies - chemistry ; Tsetse Flies - classification ; Tsetse Flies - immunology ; Tsetse Flies - physiology ; Tsetse-flies
É parte de: PLoS neglected tropical diseases, 2015-08, Vol.9 (8), p.e0004038-e0004038
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Conceived and designed the experiments: XZ TLAeS AFS LMO SA. Performed the experiments: XZ TLAeS LC AFS MO BN. Analyzed the data: XZ TLAeS AFS SA. Wrote the paper: XZ TLAeS SA.
Current address: Department of Biology, Bard College, Annandale-on-Hudson, New York, United States of America
Current address: Center for Influenza Research and Early-warning, Chinese Academy of Sciences, Beijing, China
Current address: Johns Hopkins University, Johns Hopkins Malaria Research Institute (Dept MMI), Baltimore, Maryland, United States of America
Current address: El Centro Family Health, Las Vegas, New Mexico, United States of America
The authors have declared that no competing interests exist.
Descrição: Tsetse are vectors of pathogenic trypanosomes, agents of human and animal trypanosomiasis in Africa. Components of tsetse saliva (sialome) are introduced into the mammalian host bite site during the blood feeding process and are important for tsetse's ability to feed efficiently, but can also influence disease transmission and serve as biomarkers for host exposure. We compared the sialome components from four tsetse species in two subgenera: subgenus Morsitans: Glossina morsitans morsitans (Gmm) and Glossina pallidipes (Gpd), and subgenus Palpalis: Glossina palpalis gambiensis (Gpg) and Glossina fuscipes fuscipes (Gff), and evaluated their immunogenicity and serological cross reactivity by an immunoblot approach utilizing antibodies from experimental mice challenged with uninfected flies. The protein and immune profiles of sialome components varied with fly species in the same subgenus displaying greater similarity and cross reactivity. Sera obtained from cattle from disease endemic areas of Africa displayed an immunogenicity profile reflective of tsetse species distribution. We analyzed the sialome fractions of Gmm by LC-MS/MS, and identified TAg5, Tsal1/Tsal2, and Sgp3 as major immunogenic proteins, and the 5'-nucleotidase family as well as four members of the Adenosine Deaminase Growth Factor (ADGF) family as the major non-immunogenic proteins. Within the ADGF family, we identified four closely related proteins (TSGF-1, TSGF-2, ADGF-3 and ADGF-4), all of which are expressed in tsetse salivary glands. We describe the tsetse species-specific expression profiles and genomic localization of these proteins. Using a passive-immunity approach, we evaluated the effects of rec-TSGF (TSGF-1 and TSGF-2) polyclonal antibodies on tsetse fitness parameters. Limited exposure of tsetse to mice with circulating anti-TSGF antibodies resulted in a slight detriment to their blood feeding ability as reflected by compromised digestion, lower weight gain and less total lipid reserves although these results were not statistically significant. Long-term exposure studies of tsetse flies to antibodies corresponding to the ADGF family of proteins are warranted to evaluate the role of this conserved family in fly biology.
Editor: United States: Public Library of Science
Idioma: Inglês

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Immunogenicity and Serological Cross-Reactivity of Saliva Proteins among Different Tsetse Species

Zhao, Xin ; Alves e Silva, Thiago Luiz ; Cronin, Laura ; Savage, Amy F ; O'Neill, Michelle ; Nerima, Barbara ; Okedi, Loyce M ; Aksoy, Serap Solano, Philippe

United States: Public Library of Science

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