skip to main content
Visitante
Meu Espaço
Minha Conta
Sair
Identificação
This feature requires javascript
Tags
Revistas Eletrônicas (eJournals)
Livros Eletrônicos (eBooks)
Bases de Dados
Bibliotecas USP
Ajuda
Ajuda
Idioma:
Inglês
Espanhol
Português
This feature required javascript
This feature requires javascript
Primo Search
Busca Geral
Busca Geral
Acervo Físico
Acervo Físico
Produção Intelectual da USP
Produção USP
Search For:
Clear Search Box
Search in:
Busca Geral
Or select another collection:
Search in:
Busca Geral
Busca Avançada
Busca por Índices
This feature requires javascript
This feature requires javascript
An analysis of the ramosa1 pathway in Zea mays utilizing CRISPR/Cas9 knockouts
Arndorfer, Ryan James
Iowa State University Digital Repository 2019
Sem texto completo
Citações
Citado por
Serviços
Detalhes
Resenhas & Tags
Nº de Citações
This feature requires javascript
Enviar para
Adicionar ao Meu Espaço
Remover do Meu Espaço
E-mail (máximo 30 registros por vez)
Imprimir
Link permanente
Referência
EasyBib
EndNote
RefWorks
del.icio.us
Exportar RIS
Exportar BibTeX
This feature requires javascript
Título:
An analysis of the ramosa1 pathway in Zea mays utilizing CRISPR/Cas9 knockouts
Autor:
Arndorfer, Ryan James
Assuntos:
Agriculture
;
CRISPR
;
Genetics
;
Plant Sciences
;
ramosa
;
zea mays
Notas:
Graduate Theses and Dissertations
Descrição:
Inflorescence architecture in Zea mays is affected by a large collection of interrelated genes. The ramosa genes are some of the most prominent and well-studied of these genes due to their overtly branched ear and tassel mutant phenotype. ramosa1 confers determinate, short-branch identity on branch meristems during their initiation. Several new genes are proposed to work directly with ramosa1. ail6 was identified from a Quantitative Trait Locus study to identify genes which enhance or suppress the ramosa1 branching phenotype. A Yeast-2-Hybrid study identified several genes as potential interactors with ramosa1. A CRISPR/Cas9 knockout study was performed to produce novel mutants to analyze this pathway. Two unique CRISPR expression arrays were utilized targeting 12 sites in five genes. Agrobacterium tumefaciens mediated transformation was used for transfection of Hi-II immature embryos. Both arrays functioned, and mutations were acquired in all five genes. Specifically, thirteen unique mutations have been detected in the ramosa1 gene, and in the other four genes we detected eight, 16, four and one mutation. A total of 112 plants were recovered and crossed into a B104, B73 or B73 ra1-63 background.
Editor:
Iowa State University Digital Repository
Data de criação/publicação:
2019
Idioma:
Inglês
This feature requires javascript
This feature requires javascript
Voltar para lista de resultados
This feature requires javascript
This feature requires javascript
Buscando em bases de dados remotas. Favor aguardar.
Buscando por
em
scope:(USP_PRODUCAO),scope:(USP_EBOOKS),scope:("PRIMO"),scope:(USP),scope:(USP_EREVISTAS),scope:(USP_FISICO),primo_central_multiple_fe
Mostrar o que foi encontrado até o momento
This feature requires javascript
This feature requires javascript
Página Inicial
RSS
Feed
Políticas
Fale Conosco
Logos de Redes Sociais: 
Logos de Redes Sociais: