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Ramar1, a mariner-like element in the genome of the Rhynchosciara americana

Paula Rezende Teixeira Gláucia Maria Machado-Santelli; Roberto Vicente Santelli; Congresso do Instituto de Ciências Biomédicas (4. 2005 São Paulo)

Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005

São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP 2005

Item não circula. Consulte sua biblioteca.(Acessar)

  • Título:
    Ramar1, a mariner-like element in the genome of the Rhynchosciara americana
  • Autor: Paula Rezende Teixeira
  • Gláucia Maria Machado-Santelli; Roberto Vicente Santelli; Congresso do Instituto de Ciências Biomédicas (4. 2005 São Paulo)
  • Assuntos: HISTOLOGIA
  • É parte de: Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005
  • Notas Locais: Disponível somente em CD-ROM
  • Descrição: Objectives: Transposable elements constitute a significant part of eukaryotic genome. Mariner like elements (MLE) are diverse and taxonomically wide spread group of Class II transposons that encode their own transposase, which is necessary and sufficient for transposition in the absence of host factors. They are flanked by short terminal inverted repeat (ITR elements) and a TA dinucleotide target site, which is duplicated upon insertion. The transposase consists of two domains, a N-terminal ITR binding domain and C-terminal catalytic domain. The analysis of sequences from a cDNA bank construct with mRNA of larval salivary glands of Rhynchosciara americana showed the presence of putative types of Class II elements. In the present study we describe a full-length element called Ramar1. Methods and Results: Using the inverted terminal repeat (ITR) sequences of the R. americana as primers, DNA was amplified and sequenced. A confident nucleotide consensus sequence was derived from multiple defective copies containing deletions, frame shifts and stop codons. Many conserved amino acid blocks were identified, as well as a specific D, D(34)D signature motif. Predicted transposase sequences showed 74% amino acid identity with another Sciarid, Mayetiola destructor, a known wheat pest. Southern blot analysis indicated that this transposable element is widely represented sequences in the genome of R. americana. However, Northern blot and RT-PCR analysis showed
    that this element has been expressed in different developmental stages. Phylogenetic analysis demonstrated that Ramar1 element belong to the mauritiana subfamily of mariner elements. Conclusions: The present study provides the first evidence of a full-length mariner element in the genome of the Rhynchosciara americana. These results are the beginning to understand the dynamics of MLE distribution in R.americana
  • Editor: São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP
  • Data de criação/publicação: 2005
  • Formato: res. 137.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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