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Wound-inducible nuclear protein binds DNA fragments that regulate a proteinase inhibitor II gene from potato

Palm, C.J. (Washington State University, Pullman, WA) ; Costa, M.A ; An, G ; Ryan, C.A

Proceedings of the National Academy of Sciences - PNAS, 1990-01, Vol.87 (2), p.603-607 [Periódico revisado por pares]

Washington, DC: National Academy of Sciences of the United States of America

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Título:
Wound-inducible nuclear protein binds DNA fragments that regulate a proteinase inhibitor II gene from potato
Autor: Palm, C.J. (Washington State University, Pullman, WA) ; Costa, M.A ; An, G ; Ryan, C.A
Assuntos: ADN ; Base Sequence ; Binding, Competitive ; Biological and medical sciences ; Cell Nucleus - metabolism ; Chromosome Deletion ; Cloning, Molecular ; CODE GENETIQUE ; CODIGO GENETICO ; DNA ; DNA - metabolism ; DNA-Binding Proteins - metabolism ; ENZYME INHIBITORS ; Escherichia coli - genetics ; FEUILLE ; Fundamental and applied biological sciences. Psychology ; Gels ; GENE ; Gene expression ; Gene Expression Regulation ; GENES ; GENETIC CODE ; GENETIC TRANSFORMATION ; HERIDAS ; HOJAS ; INHIBIDORES DE ENZIMAS ; INHIBITEUR D'ENZYME ; LEAVES ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutation ; Nuclear proteins ; Nuclear Proteins - biosynthesis ; Nuclear Proteins - metabolism ; NUCLEOTIDE ; NUCLEOTIDES ; NUCLEOTIDOS ; PLAIE ; Plant Proteins - biosynthesis ; Plant Proteins - metabolism ; Plants ; Plasmids ; Promoter regions ; Promoter Regions, Genetic ; PROTEASAS ; PROTEASE ; Protease inhibitors ; Protease Inhibitors - metabolism ; PROTEASES ; PROTEINAS ; PROTEINE ; PROTEINS ; Restriction Mapping ; SOLANUM TUBEROSUM ; Solanum tuberosum - genetics ; Solanum tuberosum - metabolism ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; WOUNDS
É parte de: Proceedings of the National Academy of Sciences - PNAS, 1990-01, Vol.87 (2), p.603-607
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Descrição: Deletion analysis from the 3' to the 5' end of the promoter region of the wound-inducible potato proteinase inhibitor IIK gene has identified a 421-base sequence at -136 to -557 that is necessary for expression. Utilizing DNA band-shift assays, a 10-base sequence within the 421-base region was found to bind a nuclear protein from wounded tomato leaves. This 10-base sequence is adjacent to an 8-base consensus sequence at -147 to -155 that is present in the promoter region of several elicitor-inducible genes from various other plants. The evidence suggests that a complex set of cis- and trans-acting elements within the -136 to -165 region of the potato IIK gene may be involved with the signaling mechanisms that regulate the inducibility of this gene in response to pest and pathogen attacks.
Editor: Washington, DC: National Academy of Sciences of the United States of America
Idioma: Inglês

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Wound-inducible nuclear protein binds DNA fragments that regulate a proteinase inhibitor II gene from potato

Palm, C.J. (Washington State University, Pullman, WA) ; Costa, M.A ; An, G ; Ryan, C.A

Washington, DC: National Academy of Sciences of the United States of America

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