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Compared Binding Properties of 125 I‐Labeled Analogues of Neurotensin and Neuromedin N in Rat and Mouse Brain

Gaudriault, Georges ; Zsürger, Nicole ; Vincent, Jean‐Pierre

Journal of neurochemistry, 1994-01, Vol.62 (1), p.361-368 [Periódico revisado por pares]

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  • Título:
    Compared Binding Properties of 125 I‐Labeled Analogues of Neurotensin and Neuromedin N in Rat and Mouse Brain
  • Autor: Gaudriault, Georges ; Zsürger, Nicole ; Vincent, Jean‐Pierre
  • É parte de: Journal of neurochemistry, 1994-01, Vol.62 (1), p.361-368
  • Descrição: Neurotensin and neuromedin N are two structurally related peptides that are synthesized by a common precursor. The purpose of the present work was to characterize neuromedin N receptors in rat and mouse brain and to compare these receptors with those of neurotensin. A radiolabeled analogue of neuromedin N has been prepared by acylation of the N‐terminal amino group of the peptide with the 125 I‐labeled Bolton‐Hunter reagent. This 125 I‐labeled derivative of neuromedin N bound to newborn mouse brain homogenate with high affinity (K d = 0.5 n M ). Cross‐competition experiments between radiolabeled and unlabeled neurotensin and neuromedin N indicated that each peptide was able to displace completely and specifically the other peptide from its interaction with its receptor. Independently of the radioligand used, the affinity of neurotensin was always better than that of neuromedin N. Quantitative radioautographic studies demonstrated that the ratio of labeling intensities obtained with 125 I‐labeled analogues of neurotensin and neuromedin N remained constant in all the brain areas. Our results do not support the existence of a specific neuromedin N receptor in rat and mouse brain and can be explained by the presence of a common receptor for both peptides.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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