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Inducible System for Lateral Roots in Arabidopsis thaliana and Maize
Jansen, Leen ; Parizot, Boris ; Beeckman, Tom
Methods in molecular biology (Clifton, N.J.), 2013, Vol.959, p.149-158
Totowa, NJ: Humana Press
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Título:
Inducible System for Lateral Roots in Arabidopsis thaliana and Maize
Autor:
Jansen, Leen
;
Parizot, Boris
;
Beeckman, Tom
Assuntos:
Arabidopsis - genetics
;
Arabidopsis - growth & development
;
Arabidopsis - metabolism
;
Auxin
;
Gene Expression Regulation, Plant
;
Lateral roots
;
Maize
;
Paper rolls
;
Plant Roots - genetics
;
Plant Roots - growth & development
;
Plant Roots - metabolism
;
Signal Transduction
;
Zea mays - genetics
;
Zea mays - growth & development
;
Zea mays - metabolism
É parte de:
Methods in molecular biology (Clifton, N.J.), 2013, Vol.959, p.149-158
Notas:
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição:
The study of biological processes contributing to plant growth can be complicated by the small number of cells involved and by the brief and sudden appearance of some crucial developmental steps. Given such troublesome circumstances, methods to monitor the timing or to increase the number of concerned cells can be of great advantage to researchers. Lateral root initiation is a process taking place endogenously in a discrete number of cells of the parent root. It represents the onset of the formation of a new meristem and provides the below ground part of the plant the ability to react on environmental conditions such as nutrient and water availability. Insights into the underlying mechanisms of this developmental event can be of major importance to provide means of improving tolerance to nutrient and water deficient conditions. The exact timing and site of lateral root initiation are, however, impossible to predict, hampering exhaustive studies of the sequence of events directing this process. Here, we present a method to synchronize the induction of lateral roots in Arabidopsis thaliana and maize. By initially preventing the formation of laterals in young seedlings and subsequently inducing lateral root initiation, this method not only allows controlling the process in time but also enlarges significantly the population of cells involved, opening up the way to systems biology approaches.
Títulos relacionados:
Methods in Molecular Biology
Editor:
Totowa, NJ: Humana Press
Idioma:
Inglês
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