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Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil

Bueris, Vanessa ; Sircili, Marcelo Palma ; Taddei, Carla Romano ; dos Santos, Maurilio Fernandes ; Franzolin, Marcia Regina ; Martinez, Marina Baquerizo ; Ferrer, Suzana Ramos ; Barreto, Mauricio Lima ; Trabulsi, Luiz Rachid

Memórias do Instituto Oswaldo Cruz, 2007-11, Vol.102 (7), p.839-844 [Periódico revisado por pares]

Brazil: Instituto Oswaldo Cruz, Ministério da Saúde

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Título:
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
Autor: Bueris, Vanessa ; Sircili, Marcelo Palma ; Taddei, Carla Romano ; dos Santos, Maurilio Fernandes ; Franzolin, Marcia Regina ; Martinez, Marina Baquerizo ; Ferrer, Suzana Ramos ; Barreto, Mauricio Lima ; Trabulsi, Luiz Rachid
Assuntos: Brazil ; Case-Control Studies ; Child ; Child, Preschool ; Diarrhea - microbiology ; diarrheagenic Escherichia coli ; Endemic Diseases ; Escherichia coli ; Escherichia coli - classification ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Escherichia coli Infections - microbiology ; Feces - microbiology ; Humans ; Infant ; Multiplex PCR ; PARASITOLOGY ; Polymerase Chain Reaction ; Serotyping ; TROPICAL MEDICINE ; virulence
É parte de: Memórias do Instituto Oswaldo Cruz, 2007-11, Vol.102 (7), p.839-844
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea.
Editor: Brazil: Instituto Oswaldo Cruz, Ministério da Saúde
Idioma: Inglês;Português

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Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil

Bueris, Vanessa ; Sircili, Marcelo Palma ; Taddei, Carla Romano ; dos Santos, Maurilio Fernandes ; Franzolin, Marcia Regina ; Martinez, Marina Baquerizo ; Ferrer, Suzana Ramos ; Barreto, Mauricio Lima ; Trabulsi, Luiz Rachid

Brazil: Instituto Oswaldo Cruz, Ministério da Saúde

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