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Retrospective Detection and Complete Genomic Sequencing of ICanine morbillivirus/I in Eurasian Otter Using Nanopore Technology

Lanszki, Zsófia ; Lanszki, József ; Tóth, Gábor Endre ; Zeghbib, Safia ; Jakab, Ferenc ; Kemenesi, Gábor

Viruses, 2022-06, Vol.14 (7) [Periódico revisado por pares]

MDPI AG

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  • Título:
    Retrospective Detection and Complete Genomic Sequencing of ICanine morbillivirus/I in Eurasian Otter Using Nanopore Technology
  • Autor: Lanszki, Zsófia ; Lanszki, József ; Tóth, Gábor Endre ; Zeghbib, Safia ; Jakab, Ferenc ; Kemenesi, Gábor
  • Assuntos: Analysis ; Canine distemper ; Canine distemper virus ; Care and treatment ; Diagnosis ; Diseases ; DNA sequencing ; Genetic aspects ; Growth ; Lectins ; Nucleotide sequencing ; Otters ; Phylogeny
  • É parte de: Viruses, 2022-06, Vol.14 (7)
  • Descrição: The Eurasian otter (Lutra lutra) is a piscivorous apex predator in aquatic habitats, and a flagship species of conservation biology throughout Europe. Despite the wide distribution and ecological relevance of the species, there is a considerable lack of knowledge regarding its virological and veterinary health context, especially in Central Europe. Canine morbillivirus (Canine distemper virus (CDV)) is a highly contagious viral agent of the family Paramyxoviridae with high epizootic potential and veterinary health impact. CDV is present worldwide among a wide range of animals; wild carnivores are at particular risk. As part of a retrospective study, lung-tissue samples (n = 339) from Eurasian otters were collected between 2000 and 2021 throughout Hungary. The samples were screened for CDV using a real-time RT-PCR method. Two specimens proved positive for CDV RNA. In one sample, the complete viral genome was sequenced using a novel, pan-genotype CDV-specific amplicon-based sequencing method with Oxford Nanopore sequencing technology. Both viral sequences were grouped to a European lineage based on the hemagglutinin-gene phylogenetic classification. In this article, we present the feasibility of road-killed animal samples for understanding the long-term dynamics of CDV among wildlife and provide novel virological sequence data to better understand CDV circulation and evolution.
  • Editor: MDPI AG
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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