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Inhaled Crocidolite Mutagenicity in Lung DNA

Rihn, Bertrand ; Coulais, Catherine ; Kauffer, Edmond ; Bottin, Marie-Claire ; Martin, Patrick ; Yvon, Francois ; Vigneron, Jean Claude ; Binet, Stéphane ; Monhoven, Nathalie ; Steiblen, Guy ; Keith, Gérard

Environmental health perspectives, 2000-04, Vol.108 (4), p.341-346 [Periódico revisado por pares]

United States: National Institute of Environmental Health Sciences. National Institutes of Health. Department of Health, Education and Welfare

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Título:
Inhaled Crocidolite Mutagenicity in Lung DNA
Autor: Rihn, Bertrand ; Coulais, Catherine ; Kauffer, Edmond ; Bottin, Marie-Claire ; Martin, Patrick ; Yvon, Francois ; Vigneron, Jean Claude ; Binet, Stéphane ; Monhoven, Nathalie ; Steiblen, Guy ; Keith, Gérard
Assuntos: Adducts ; Aerosols ; Air Pollutants - adverse effects ; Animals ; Asbestos ; Asbestos, Crocidolite - administration & dosage ; Asbestos, Crocidolite - adverse effects ; DNA ; DNA Adducts - genetics ; DNA Damage - genetics ; Dosage ; Genetic mutation ; Inhalation ; Inhalation Exposure ; Lung - drug effects ; Lung - pathology ; Lungs ; Macrophages, Alveolar - physiology ; Male ; Mice ; Mice, Transgenic ; Mutagenicity ; Mutagenicity Tests ; Transgenic animals
É parte de: Environmental health perspectives, 2000-04, Vol.108 (4), p.341-346
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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Descrição: We used transgenic mice carrying the lacI reporter gene to study the mutagenesis potential of asbestos crocidolite. The animals were exposed by nose-only inhalation to an aerosol containing 5.75 mg/ m3crocidolite dust for 6 hr/day and 5 consecutive days. After 1, 4, and 12 weeks, we examined four end points: the cytology of bronchoalveolar lavage, the lung load of crocidolite, the hydrophobic DNA adducts, and the mutations in the lacI reporter gene. Twelve weeks after exposure, nearly 10% of the inhaled fibers remained in the lung (227 ± 103 ng/mg lung). There was evidence of a typical inflammatory response consisting of multinucleate macrophages at weeks 4 and 12, whereas immediately after the exposure, we observed numerous polymorphonuclear neutrophils. The mutant frequency significatively increased during the fourth week after the exposure: 13.5× 10-5in the exposed group versus 6.9× 10-5in the control group. The induction factor, defined by the ratio of checked mutants of exposed mice to checked mutants of control mice, was 1.96. The mutation spectrum of control lung DNA and exposed lung DNA was similar, suggesting the possible involvement of a DNA repair decrease in crocidolite-treated animals. We used the32P-postlabeling method and did not detect any increase of either 5 mC or bulky adduct in treated mice. This is the first study that demonstrates asbestos mutagenicity in vivo after a nose-only inhalation.
Editor: United States: National Institute of Environmental Health Sciences. National Institutes of Health. Department of Health, Education and Welfare
Idioma: Inglês

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Inhaled Crocidolite Mutagenicity in Lung DNA

Rihn, Bertrand ; Coulais, Catherine ; Kauffer, Edmond ; Bottin, Marie-Claire ; Martin, Patrick ; Yvon, Francois ; Vigneron, Jean Claude ; Binet, Stéphane ; Monhoven, Nathalie ; Steiblen, Guy ; Keith, Gérard

United States: National Institute of Environmental Health Sciences. National Institutes of Health. Department of Health, Education and Welfare

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