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Detection of β-amyloid peptide (1–16) and amyloid precursor protein (APP 770) using spectroscopic ellipsometry and QCM techniques: A step forward towards Alzheimers disease diagnostics

Mustafa, M.K. ; Nabok, A. ; Parkinson, D. ; Tothill, I.E. ; Salam, F. ; Tsargorodskaya, A.

Biosensors & bioelectronics, 2010-12, Vol.26 (4), p.1332-1336 [Periódico revisado por pares]

Kidlington: Elsevier B.V

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Título:
Detection of β-amyloid peptide (1–16) and amyloid precursor protein (APP 770) using spectroscopic ellipsometry and QCM techniques: A step forward towards Alzheimers disease diagnostics
Autor: Mustafa, M.K. ; Nabok, A. ; Parkinson, D. ; Tothill, I.E. ; Salam, F. ; Tsargorodskaya, A.
Assuntos: Alzheimer Disease - diagnosis ; Alzheimer Disease - metabolism ; Alzheimer's disease ; Amyloid beta-Peptides - analysis ; Amyloid beta-Peptides - immunology ; Amyloid beta-Protein Precursor - analysis ; Amyloid beta-Protein Precursor - immunology ; Amyloid precursor protein (APP 770) ; Animals ; Antibodies, Immobilized ; Antibodies, Monoclonal - metabolism ; Antigen-Antibody Reactions ; Biological and medical sciences ; Biotechnology ; Direct immunoassay ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoassay - methods ; In Vitro Techniques ; Kinetics ; Mice ; Peptide Fragments - analysis ; Peptide Fragments - immunology ; Quartz crystal microbalance ; Quartz Crystal Microbalance Techniques - methods ; Quartz Crystal Microbalance Techniques - statistics & numerical data ; Spectrum Analysis - methods ; Surface Plasmon Resonance - methods ; Total internal reflection ellipsometry ; β-Amyloid peptide 1–16
É parte de: Biosensors & bioelectronics, 2010-12, Vol.26 (4), p.1332-1336
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SourceType-Scholarly Journals-1
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Descrição: A highly sensitive method of spectroscopic ellipsometry in total internal reflection mode (TIRE) was exploited for detecting β-amyloid peptide (Aβ 1–16) in the direct immune reaction with monoclonal DE2 antibodies (raised against Aβ 1–16) electrostatically immobilised on the surface of gold. A rapid detection of Aβ 1–16 in a wide range of concentrations from 5 μg/ml down to 0.05 ng/ml was achieved using a cost-effective and label-free direct immunoassay format. TIRE dynamic spectral measurements proved that the immune reaction between DE2 monoclonal antibodies and Aβ 1–16 is highly specific with the affinity constant K D = 1.46 × 10 −8 mol/l . The same DE2 antibodies were utilised for detection of amyloid precursor protein APP 770, a larger protein containing Aβ 1–16 domain, using the quartz crystal microbalance (QCM) measurements in liquid. A combination of QCM and TIRE kinetics results allowed the evaluation of the originally unknown concentration of APP 770 in complete medium solution containing other proteins, salts, and amino acids.
Editor: Kidlington: Elsevier B.V
Idioma: Inglês

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Detection of β-amyloid peptide (1–16) and amyloid precursor protein (APP 770) using spectroscopic ellipsometry and QCM techniques: A step forward towards Alzheimers disease diagnostics

Mustafa, M.K. ; Nabok, A. ; Parkinson, D. ; Tothill, I.E. ; Salam, F. ; Tsargorodskaya, A.

Kidlington: Elsevier B.V

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