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Detection of low molecular weight toxins using optical phase detection techniques

Nabok, A. ; Tsargorodskaya, A. ; Mustafa, M.K. ; Székács, A. ; Székács, I. ; Starodub, N.F.

Procedia chemistry, 2009-09, Vol.1 (1), p.1491-1494 [Periódico revisado por pares]

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Título:
Detection of low molecular weight toxins using optical phase detection techniques
Autor: Nabok, A. ; Tsargorodskaya, A. ; Mustafa, M.K. ; Székács, A. ; Székács, I. ; Starodub, N.F.
Assuntos: direct immunoassay ; mycotoxins ; planar waveguide ; polarization interferometry ; total internal reflection ellipsometry
É parte de: Procedia chemistry, 2009-09, Vol.1 (1), p.1491-1494
Descrição: Optical methods based on recording a phase shift of electromagnetic waves offer high sensitivity for detection of bio-reactions. The method of Total Internal Reflection Ellipsometry (TIRE), which records simultaneously two parameters Ψ and Δ related, respectively, to the amplitudes and phases of p- and s-components of polarized light, was exploited here for detection of mycotoxins (T-2, zearalanone, and aflatoxin). The TIRE direct label-free immunoassay enables detecting the above mycotoxins in sub-ppb concentrations in water as well as in food extracts. Further enhancement of sensitivity can be achieved with the use of SiO2/Si3N4/SiO2 planar waveguides operating as polarization interferometers (PI).
Editor: Elsevier B.V
Idioma: Inglês

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