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On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development

Peshkin, Leonid ; Wühr, Martin ; Pearl, Esther ; Haas, Wilhelm ; Freeman, Robert M. ; Gerhart, John C. ; Klein, Allon M. ; Horb, Marko ; Gygi, Steven P. ; Kirschner, Marc W.

Developmental cell, 2015-11, Vol.35 (3), p.383-394 [Periódico revisado por pares]

United States: Elsevier Inc

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  • Título:
    On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development
  • Autor: Peshkin, Leonid ; Wühr, Martin ; Pearl, Esther ; Haas, Wilhelm ; Freeman, Robert M. ; Gerhart, John C. ; Klein, Allon M. ; Horb, Marko ; Gygi, Steven P. ; Kirschner, Marc W.
  • Assuntos: Aging ; Animals ; Embryonic Development - genetics ; Gene Expression Regulation, Developmental - genetics ; Protein Biosynthesis - genetics ; Proteome - genetics ; RNA, Messenger - genetics ; Transcription, Genetic - genetics ; Xenopus laevis - embryology ; Xenopus laevis - metabolism ; Xenopus Proteins - metabolism
  • É parte de: Developmental cell, 2015-11, Vol.35 (3), p.383-394
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: A biochemical explanation of development from the fertilized egg to the adult requires an understanding of the proteins and RNAs expressed over time during embryogenesis. We present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus. Surprisingly, we find that most protein levels change little and duplicated genes are expressed similarly. While the correlation between protein and mRNA levels is poor, a mass action kinetics model parameterized using protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration. This study provides detailed data for absolute levels of ∼10,000 proteins and ∼28,000 transcripts via a convenient web portal, a rich resource for developmental biologists. It underscores the lasting impact of maternal dowry, finds surprisingly few cases where degradation alone drives a change in protein level, and highlights the importance of transcription in shaping the dynamics of the embryonic proteome. [Display omitted] •A genome-scale resource of mRNA and protein expression for vertebrate embryogenesis•Temporal patterns of change in mRNA and protein abundance are poorly correlated•A simple kinetic model explains protein expression as a function of mRNA levels•Embryogenesis is driven by maternal protein dowry and tissue-specific transcription Embryos express proteins at the correct time during development, by balancing the maternal contribution with protein synthesis and degradation. Peshkin et al. determine the absolute concentrations of ∼10,000 proteins and ∼28,000 transcripts across Xenopus development, uncovering the relative roles of these three processes across the proteome and revealing global trends.
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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