Idioma:

Comparison of specific radioactivities of human α-lactalbumin iodinated by three different methods

Thean, Eric T.

Analytical biochemistry, 1990-08, Vol.188 (2), p.330-334 [Periódico revisado por pares]

United States: Elsevier Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Comparison of specific radioactivities of human α-lactalbumin iodinated by three different methods
Autor: Thean, Eric T.
Assuntos: 550201 - Biochemistry- Tracer Techniques ; ALBUMINS ; alpha -lactalbumin ; ANIMALS ; ANTIBODIES ; Antibodies, Monoclonal ; BASIC BIOLOGICAL SCIENCES ; BIOASSAY ; CHEMICAL REACTIONS ; Chloramines - metabolism ; choramine-T ; DIAGNOSTIC TECHNIQUES ; ENZYMES ; HALOGENATION ; Humans ; IMMUNOASSAY ; IMMUNOLOGY ; Indicators and Reagents ; IODINATION ; IODINE ISOTOPES ; Iodine Radioisotopes ; Iodogen ; ISOTOPE APPLICATIONS ; Isotope Labeling ; ISOTOPES ; Lactalbumin - analysis ; Lactalbumin - immunology ; Lactalbumin - metabolism ; lactoperoxidase ; Lactoperoxidase - metabolism ; MAMMALS ; MAN ; MONOCLONAL ANTIBODIES ; ORGANIC COMPOUNDS ; OXIDOREDUCTASES ; PEROXIDASES ; Precipitin Tests ; PRIMATES ; PROTEINS ; RADIOASSAY ; RADIOIMMUNOASSAY ; Radioimmunoassay - methods ; RADIOIMMUNODETECTION ; RADIOIMMUNOLOGY ; Tosyl Compounds ; TRACER TECHNIQUES ; Urea - analogs & derivatives ; Urea - metabolism ; VERTEBRATES
É parte de: Analytical biochemistry, 1990-08, Vol.188 (2), p.330-334
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Radioiodination provides an extremely sensitive method for the detection of low levels of proteins. In the development of a sensitive radioimmunoassay for human α-lactalbumin (α-LA), the protein was labeled to high specific activity (approaching 2000 Ci/mmol) with lactoperoxidase, chloramine-T, and Iodogen. Despite high specific activities of the labeled protein by each method, there was a considerable difference in their binding affinity with monoclonal anti-human α-LA antibodies due to varying degrees of protein damage. Iodination of human α-LA with Iodogen resulted in labels of the highest specific activity and immunoreactivity with the monoclonal antibodies used.
Editor: United States: Elsevier Inc
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Comparison of specific radioactivities of human α-lactalbumin iodinated by three different methods

Thean, Eric T.

United States: Elsevier Inc

Buscando em bases de dados remotas. Favor aguardar.