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A Bifunctional Delta 12, Delta 15-Desaturase from Acanthamoeba castellanii Directs the Synthesis of Highly Unusual n-1 Series Unsaturated Fatty Acids

Sayanova, Olga ; Haslam, Richard ; Guschina, Irina ; Lloyd, David ; Christie, William W ; Harwood, John L ; Napier, Johnathan A

The Journal of biological chemistry, 2006-12, Vol.281 (48), p.36533-36541 [Periódico revisado por pares]

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  • Título:
    A Bifunctional Delta 12, Delta 15-Desaturase from Acanthamoeba castellanii Directs the Synthesis of Highly Unusual n-1 Series Unsaturated Fatty Acids
  • Autor: Sayanova, Olga ; Haslam, Richard ; Guschina, Irina ; Lloyd, David ; Christie, William W ; Harwood, John L ; Napier, Johnathan A
  • Assuntos: Acanthamoeba castellanii
  • É parte de: The Journal of biological chemistry, 2006-12, Vol.281 (48), p.36533-36541
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
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  • Descrição: The free-living soil protozoon Acanthamoeba castellanii synthesizes a range of polyunsaturated fatty acids, the balance of which can be altered by environmental changes. We have isolated and functionally characterized in yeast a microsomal desaturase from A. castellanii, which catalyzes the sequential conversion of C sub(16) and C sub(18) Delta 9-monounsaturated fatty acids to di- and tri-unsaturated forms. In the case of C sub(16) substrates, this bifunctional A. castellanii Delta 12, Delta 15-desaturase generated a highly unusual fatty acid, hexadecatrienoic acid (16:3 Delta super(9,12,15)(n-1)). The identification of a desaturase, which can catalyze the insertion of a double bond between the terminal two carbons of a fatty acid represents a new addition to desaturase functionality and plasticity. We have also co-expressed in yeast the A. castellanii bifunctional Delta 12, Delta 15-desaturase with a microsomal Delta 6-desaturase, resulting in the synthesis of the highly unsaturated C sub(16) fatty acid hexadecatetraenoic acid (16:4 Delta super(6,9,12,15)(n-1)), previously only reported in marine microorganisms. Our work therefore demonstrates the feasibility of the heterologous synthesis of polyunsaturated fatty acids of the n-1 series. The presence of a bifunctional Delta 12, Delta 15-desaturase in A. castellanii is also considered with reference to the evolution of desaturases and the lineage of this protist.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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