Idioma:

Distinct carbon sources affect structural and functional maturation of cardiomyocytes derived from human pluripotent stem cells

Correia, Cláudia ; Koshkin, Alexey ; Duarte, Patrícia ; Hu, Dongjian ; Teixeira, Ana ; Domian, Ibrahim ; Serra, Margarida ; Alves, Paula M

Scientific reports, 2017-08, Vol.7 (1), p.8590-17, Article 8590 [Periódico revisado por pares]

England: Nature Publishing Group

Texto completo disponível

Citações Citado por

Enviar para

Título:
Distinct carbon sources affect structural and functional maturation of cardiomyocytes derived from human pluripotent stem cells
Autor: Correia, Cláudia ; Koshkin, Alexey ; Duarte, Patrícia ; Hu, Dongjian ; Teixeira, Ana ; Domian, Ibrahim ; Serra, Margarida ; Alves, Paula M
Assuntos: Action potential ; Biomarkers - metabolism ; Calcium ; Calcium - metabolism ; Carbon - pharmacology ; Carbon sources ; Cardiomyocytes ; Cell culture ; Cell Differentiation - drug effects ; Cells, Cultured ; Contractility ; Culture Media, Conditioned - pharmacology ; Fatty acids ; Fatty Acids - toxicity ; Galactose ; Galactose - pharmacology ; Glucose - deficiency ; Glycolysis - drug effects ; Heart Ventricles - cytology ; Humans ; Kinetics ; Lactose - pharmacology ; Models, Biological ; Myocytes, Cardiac - drug effects ; Myocytes, Cardiac - metabolism ; Oxidation ; Oxidation-Reduction ; Oxidative metabolism ; Oxidative Phosphorylation - drug effects ; Pluripotency ; Pluripotent Stem Cells - drug effects ; Pluripotent Stem Cells - metabolism ; Pluripotent Stem Cells - ultrastructure ; Stem cells ; Structure-function relationships ; Transcription ; Transcription, Genetic - drug effects ; Transcriptome - genetics ; Ventricle
É parte de: Scientific reports, 2017-08, Vol.7 (1), p.8590-17, Article 8590
Descrição: The immature phenotype of human pluripotent stem cell derived cardiomyocytes (hPSC-CMs) constrains their potential in cell therapy and drug testing. In this study, we report that shifting hPSC-CMs from glucose-containing to galactose- and fatty acid-containing medium promotes their fast maturation into adult-like CMs with higher oxidative metabolism, transcriptional signatures closer to those of adult ventricular tissue, higher myofibril density and alignment, improved calcium handling, enhanced contractility, and more physiological action potential kinetics. Integrated "-Omics" analyses showed that addition of galactose to culture medium improves total oxidative capacity of the cells and ameliorates fatty acid oxidation avoiding the lipotoxicity that results from cell exposure to high fatty acid levels. This study provides an important link between substrate utilization and functional maturation of hPSC-CMs facilitating the application of this promising cell type in clinical and preclinical applications.
Editor: England: Nature Publishing Group
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Distinct carbon sources affect structural and functional maturation of cardiomyocytes derived from human pluripotent stem cells

Correia, Cláudia ; Koshkin, Alexey ; Duarte, Patrícia ; Hu, Dongjian ; Teixeira, Ana ; Domian, Ibrahim ; Serra, Margarida ; Alves, Paula M

England: Nature Publishing Group

Buscando em bases de dados remotas. Favor aguardar.