skip to main content
Visitante
Meu Espaço
Minha Conta
Sair
Identificação
This feature requires javascript
Tags
Revistas Eletrônicas (eJournals)
Livros Eletrônicos (eBooks)
Bases de Dados
Bibliotecas USP
Ajuda
Ajuda
Idioma:
Inglês
Espanhol
Português
This feature required javascript
This feature requires javascript
Primo Search
Busca Geral
Busca Geral
Acervo Físico
Acervo Físico
Produção Intelectual da USP
Produção USP
Search For:
Clear Search Box
Search in:
Busca Geral
Or hit Enter to replace search target
Or select another collection:
Search in:
Busca Geral
Busca Avançada
Busca por Índices
This feature requires javascript
This feature requires javascript
Investigation into the Relationship Between DNA Content and DNA Yield Prediction and Presence of a Multiple Contributor Cell Population in Trace DNA
Roisch, Sarah Holton
VCU Scholars Compass 2023
Texto completo disponível
Citações
Citado por
Exibir Online
Detalhes
Resenhas & Tags
Mais Opções
Nº de Citações
This feature requires javascript
Enviar para
Adicionar ao Meu Espaço
Remover do Meu Espaço
E-mail (máximo 30 registros por vez)
Imprimir
Link permanente
Referência
EasyBib
EndNote
RefWorks
del.icio.us
Exportar RIS
Exportar BibTeX
This feature requires javascript
Título:
Investigation into the Relationship Between DNA Content and DNA Yield Prediction and Presence of a Multiple Contributor Cell Population in Trace DNA
Autor:
Roisch, Sarah Holton
Assuntos:
Forensic Science and Technology
Notas:
https://scholarscompass.vcu.edu/frsc_projects/65
Master of Science in Forensic Science Directed Research Projects
https://scholarscompass.vcu.edu/context/frsc_projects/article/1066/viewcontent/Sarah_Holton_Roisch_Thesis_May_2023.pdf
Descrição:
Due to advancements in DNA analysis technology and an increased ability to detect trace quantities of low template DNA, ‘touch’ or trace DNA has become a prevalent form of evidence for forensic investigations. Trace DNA is biological material left on a surface from skin cells after being touched or handled. The success rate of casework could be improved with new methods to non-destructively screen trace DNA to predict DNA yield and whether multiple cell populations are present in the evidence prior to STR analysis. Current methods of trace DNA analysis require quantifying the sample with qPCR followed by STR analysis, however, low amounts of template DNA may cause allelic drop-out or results in uninterpretable profiles both of which can reduce or even eliminate the probative value of the evidence. Androgens, such as testosterone, make a feasible biological marker for pre-DNA analysis trace samples due to varied secretion levels between individuals and possible persistence in the epidermis. Another potential target is cytokeratin molecules which are a diverse and abundant class of proteins found throughout epidermal cells. This study surveyed variation in the abundance of either testosterone or cytokeratin antibodies in shed epidermal cells collected through direct swabs of individuals’ hands. Fluorescently tagged antibodies of either cytokeratin or testosterone were hybridized to the epidermal cells and then analyzed using flow cytometry followed by DNA analysis. The results demonstrated that although cytokeratin AE3 binding efficacy varied between individual donors, no systematic relationship was observed between probe binding and DNA yield or the presence of multiple contributor cell populations. Testosterone-stained contact samples also exhibited differences between some pairs of contributor cell populations, but there was not a clear quantitative relationship between probe binding and the presence of multiple contributors in the sample. However, testosterone abundance did show some evidence of a correlation to DNA yield (R2 = 0.66) and a stronger correlation when high yield (>1 ng) DNA samples were excluded (R2 = 0.90). Analysis of unstained contact cells showed the presence of nucleated epithelial cells in touch samples indicating a possible source of DNA in trace evidence. These results may provide a novel method for front-end analysis of trace samples pre-DNA characterization using testosterone to estimate DNA content. Additionally, the variability in antibody binding efficacy may be due to the heterozygosity within touch samples as cellular composition varied between individuals and further investigation could be to exploit these differences for distinguishing between multiple contributors. The ability to non-destructively triage and characterize trace DNA evidence prior to DNA profiling would help address the challenges associated with touch evidence to conserve valuable time and resources.
Editor:
VCU Scholars Compass
Data de criação/publicação:
2023
Idioma:
Inglês
Links
View record in Virginia Commonwealth University$$FView record in $$GVirginia Commonwealth University
This feature requires javascript
This feature requires javascript
Voltar para lista de resultados
This feature requires javascript
This feature requires javascript
Buscando em bases de dados remotas. Favor aguardar.
Buscando por
em
scope:(USP_VIDEOS),scope:("PRIMO"),scope:(USP_FISICO),scope:(USP_EREVISTAS),scope:(USP),scope:(USP_EBOOKS),scope:(USP_PRODUCAO),primo_central_multiple_fe
Mostrar o que foi encontrado até o momento
This feature requires javascript
This feature requires javascript