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Molecular characterization and tissue‐specific gene expression of Dermacentor variabilisα‐catenin in response to rickettsial infection

Sunyakumthorn, P ; Petchampai, N ; Kearney, M. T ; Sonenshine, D. E ; Macaluso, K. R

Insect molecular biology, 2012-04, Vol.21 (2), p.197-204 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Molecular characterization and tissue‐specific gene expression of Dermacentor variabilisα‐catenin in response to rickettsial infection
Autor: Sunyakumthorn, P ; Petchampai, N ; Kearney, M. T ; Sonenshine, D. E ; Macaluso, K. R
Assuntos: Actin ; alpha -catenin ; alpha Catenin - genetics ; alpha Catenin - metabolism ; Amino Acid Sequence ; Animals ; Arthropod Proteins - genetics ; Arthropod Proteins - metabolism ; Arthropod Vectors - genetics ; Arthropod Vectors - metabolism ; cadherins ; catenin ; cell adhesion ; complementary DNA ; cytoskeletal proteins ; Cytoskeleton ; Dermacentor ; Dermacentor - genetics ; Dermacentor - metabolism ; Dermacentor - microbiology ; Dermacentor variabilis ; E-Cadherin ; Feeding ; Feeding Behavior ; Female ; Gene Expression ; Infection ; Ixodes ; Ixodes scapularis ; Ixodidae ; Molecular Sequence Data ; Open reading frames ; Ovaries ; Polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; Rickettsia ; Rickettsia - physiology ; Rickettsia amblyommii ; Rickettsia Infections - transmission ; Rickettsia montanensis ; rickettsial diseases ; RNA, Messenger - metabolism ; sequence analysis ; Sequence Analysis, DNA ; ticks ; α-catenin
É parte de: Insect molecular biology, 2012-04, Vol.21 (2), p.197-204
Notas: http://dx.doi.org/10.1111/j.1365-2583.2011.01126.x
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Descrição: Alpha catenin is a cytoskeleton protein that acts as a regulator of actin rearrangement by forming an E‐cadherin adhesion complex. In Dermacentor variabilis, a putative α‐catenin (Dvα‐catenin) was previously identified as differentially regulated in ovaries of ticks chronically infected with Rickettsia montanensis. To begin characterizing the role(s) of Dvα‐catenin during rickettsial infection, the full‐length Dvα‐catenin cDNA was cloned and analysed. Comparative sequence analysis demonstrates a 3069‐bp cDNA with a 2718‐bp open reading frame with a sequence similar to Ixodes scapularisα‐catenin. A portion of Dvα‐catenin is homologous to the vinculin‐conserved domain containing a putative actin‐binding region and β‐catenin‐binding and ‐dimerization regions. Quantitative reverse‐transcription PCR analysis demonstrated that Dvα‐catenin is predominantly expressed in tick ovaries and is responsive to tick feeding. The tissue‐specific gene expression analysis of ticks exposed to Rickettsia demonstrates that Dvα‐catenin expression was significantly downregulated 12 h after exposure to R. montanensis, but not in Rickettsia amblyommii‐exposed ovaries, compared with Rickettsia‐unexposed ticks. Studying tick‐derived molecules associated with rickettsial infection will provide a better understanding of the transmission dynamics of tick‐borne rickettsial diseases.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Molecular characterization and tissue‐specific gene expression of Dermacentor variabilisα‐catenin in response to rickettsial infection

Sunyakumthorn, P ; Petchampai, N ; Kearney, M. T ; Sonenshine, D. E ; Macaluso, K. R

Oxford, UK: Blackwell Publishing Ltd

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