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A critical evaluation of ultrasensitive single-cell proteomics strategies

Nalehua, Mary Rachel ; Zaia, Joseph

Analytical and bioanalytical chemistry, 2024-04, Vol.416 (9), p.2359-2369 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
A critical evaluation of ultrasensitive single-cell proteomics strategies
Autor: Nalehua, Mary Rachel ; Zaia, Joseph
Assuntos: Advances in (Bio-)Analytical Chemistry: Reviews and Trends Collection 2024 ; Alternative splicing ; Analytical Chemistry ; Biochemistry ; Biology ; Biomedical research ; Cells ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Critical Review ; Data analysis ; Food Science ; Genomes ; Genomics ; Heterogeneity ; Instrumentation ; Labeling ; Laboratory Medicine ; Mass spectrometry ; Mass Spectrometry - methods ; Mass spectroscopy ; Monitoring/Environmental Analysis ; Peptides ; Phosphorylation ; Post-translation ; Protein Processing, Post-Translational ; Proteins ; Proteome - analysis ; Proteomes ; Proteomics ; Proteomics - methods ; Sample preparation ; Scientific imaging ; Signal quality ; Tradeoffs ; Transcriptomics
É parte de: Analytical and bioanalytical chemistry, 2024-04, Vol.416 (9), p.2359-2369
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
Descrição: Success of mass spectrometry characterization of the proteome of single cells allows us to gain a greater understanding than afforded by transcriptomics alone but requires clear understanding of the tradeoffs between analytical throughput and precision. Recent advances in mass spectrometry acquisition techniques, including updated instrumentation and sample preparation, have improved the quality of peptide signals obtained from single cell data. However, much of the proteome remains uncharacterized, and higher throughput techniques often come at the expense of reduced sensitivity and coverage, which diminish the ability to measure proteoform heterogeneity, including splice variants and post-translational modifications, in single cell data analysis. Here, we assess the growing body of ultrasensitive single-cell approaches and their tradeoffs as researchers try to balance throughput and precision in their experiments.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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A critical evaluation of ultrasensitive single-cell proteomics strategies

Nalehua, Mary Rachel ; Zaia, Joseph

Berlin/Heidelberg: Springer Berlin Heidelberg

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