skip to main content
Idioma:
Primo Search
Clear Search Box
Search in: Busca Geral
Busca Avançada
Busca por Índices
Tipo de recurso Mostra resultados com: Mostra resultados com: Índice

Downregulation of hTERT: An Important As.sub.2O.sub.3 Induced Mechanism of Apoptosis in Myelodysplastic Syndrome.(Research Article)

Xu, Weilai ; Wang, Yungui ; Tong, Hongyan ; Qian, Wenbin ; Jin, Jie

PloS one, 2014-11, Vol.9 (11) [Periódico revisado por pares]

Public Library of Science

Texto completo disponível

Citações Citado por
  • Título:
    Downregulation of hTERT: An Important As.sub.2O.sub.3 Induced Mechanism of Apoptosis in Myelodysplastic Syndrome.(Research Article)
  • Autor: Xu, Weilai ; Wang, Yungui ; Tong, Hongyan ; Qian, Wenbin ; Jin, Jie
  • Assuntos: Apoptosis ; Arsenic ; Arsenic trioxide ; Enzymes
  • É parte de: PloS one, 2014-11, Vol.9 (11)
  • Descrição: Two myelodysplastic syndrome (MDS) celllines, MUTZ-1 and SKM-1 cells, were used to study the effect of arsenic trioxide (As.sub.2 O.sub.3) on hematological malignant cells. As.sub.2 O.sub.3 induced this two cell lines apoptosis via activation of caspase-3/8 and cleavage of poly (ADP-ribose) polymerase (PARP), a DNA repair enzyme. As.sub.2 O.sub.3 reduced NF-[kappa]B activity, which was important for inducing MUTZ-1 and SKM-1 cells apoptosis. As.sub.2 O.sub.3 also inhibited the activities of hTERT in MUTZ-1 and SKM-1 cells. Moreover, the NF-[kappa]B inhibitor, pyrrolidine dithiocarbamate (PDTC), had no effect on caspase-8 activation, although PDTC did inhibit MUTZ-1 and SKM-1 cells proliferation. Incubation of MUTZ-1 cells with a caspase-8 inhibitor failed to block As.sub.2 O.sub.3 -induced inhibition of NF-[kappa]B activity. Our findings suggest that As.sub.2 O.sub.3 may induce apoptosis in MUTZ-1 and SKM-1 cells by two independent pathways: first, by activation of caspase-3/8 and PARP; and second, by inhibition of NF-[kappa]B activity, which results in downregulation of hTERT expression. We conclude that hTERT and NF-[kappa]B are important molecular targets in As.sub.2 O.sub.3 -induced apoptosis.
  • Editor: Public Library of Science
  • Idioma: Inglês
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.