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Refinado por: Nome da Publicação: Biotechniques remover assunto: Genetic Engineering remover
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New cloning vectors to facilitate quick allelic exchange in gram-negative bacteria
New cloning vectors to facilitate quick allelic exchange in gram-negative bacteria
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New cloning vectors to facilitate quick allelic exchange in gram-negative bacteria

Mejia-Santana, Adrian ; Lloyd, Cameron J ; Klose, Karl E

BioTechniques, 2021-02, Vol.70 (2), p.116-119 [Periódico revisado por pares]

England: Future Science Ltd

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2
Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes
Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes
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Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes

Wang, Bangmei ; Li, Kunyu ; Wang, Amy ; Reiser, Michelle ; Saunders, Thom ; Lockey, Richard F ; Wang, Jia-Wang

BioTechniques, 2015-10, Vol.59 (4), p.201-208 [Periódico revisado por pares]

England: Future Science Ltd

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3
Two-step Red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
Two-step Red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
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Two-step Red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli

TISCHER, B. Karsten ; VON EINEM, Jens ; KAUFER, Benedikt ; OSTERRIEDER, Nikolaus

BioTechniques, 2006-02, Vol.40 (2), p.191-197 [Periódico revisado por pares]

Natick, MA: Eaton

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4
Synthetic biology: engineering order in organisms across scales and species
Synthetic biology: engineering order in organisms across scales and species
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Synthetic biology: engineering order in organisms across scales and species

Jia, Bill ; Wongprommoon, Arin

BioTechniques, 2018-09, Vol.65 (3), p.113-119 [Periódico revisado por pares]

England: Future Science Ltd

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5
Processing of gene expression data generated by quantitative real-time RT-PCR
Processing of gene expression data generated by quantitative real-time RT-PCR
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Processing of gene expression data generated by quantitative real-time RT-PCR

MULLER, Patrick Y ; JANOVJAK, Harald ; MISEREZ, André R ; DOBBIE, Zuzana

BioTechniques, 2002-06, Vol.32 (6), p.1372-1379 [Periódico revisado por pares]

Natick, MA: Eaton

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6
A useful gene cassette for conditional knock-down of essential genes by targeted promoter replacement in Mycobacteria
A useful gene cassette for conditional knock-down of essential genes by targeted promoter replacement in Mycobacteria
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A useful gene cassette for conditional knock-down of essential genes by targeted promoter replacement in Mycobacteria

Texier, Pauline ; Coddeville, Michèle ; Bordes, Patricia ; Genevaux, Pierre

BioTechniques, 2018-09, Vol.65 (3), p.159-162 [Periódico revisado por pares]

England: Future Science Ltd

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7
PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic C. elegans
PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic C. elegans
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PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic C. elegans

HOBERT, Oliver

BioTechniques, 2002-04, Vol.32 (4), p.728-730 [Periódico revisado por pares]

Natick, MA: Eaton

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8
Semi-automated Tip Snip cloning of restriction fragments into and out of plasmid polylinkers
Semi-automated Tip Snip cloning of restriction fragments into and out of plasmid polylinkers
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Semi-automated Tip Snip cloning of restriction fragments into and out of plasmid polylinkers

Matsumura, Ichiro

BioTechniques, 2017-03, Vol.62 (3), p.99-106 [Periódico revisado por pares]

England: Future Science Ltd

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9
Combined sacB-based negative selection and cre-lox antibiotic marker recycling for efficient gene deletion in Pseudomonas aeruginosa
Combined sacB-based negative selection and cre-lox antibiotic marker recycling for efficient gene deletion in Pseudomonas aeruginosa
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Combined sacB-based negative selection and cre-lox antibiotic marker recycling for efficient gene deletion in Pseudomonas aeruginosa

QUENEE, Lauriane ; LAMOTTE, Danièle ; POLACK, Benoit

BioTechniques, 2005, Vol.38 (1), p.63-67 [Periódico revisado por pares]

Natick, MA: Eaton

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10
Quantitative RT-PCR: Pitfalls and Potential
Quantitative RT-PCR: Pitfalls and Potential
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Quantitative RT-PCR: Pitfalls and Potential

Freeman, Willard M ; Walker, Stephen J ; Vrana, Kent E

BioTechniques, 1999-01, Vol.26 (1), p.112-125 [Periódico revisado por pares]

Natick, MA: Future Science Ltd

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