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Activation of 5-HT 4 receptors facilitates neurogenesis from transplanted neural stem cells in the anastomotic ileum

Goto, Kei ; Kawahara, Isao ; Inada, Hiroyuki ; Misawa, Hiromi ; Kuniyasu, Hiroki ; Nabekura, Junich ; Takaki, Miyako

The Journal of Physiological Sciences, 2016, Vol.66(1), pp.67-76 [Periódico revisado por pares]

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  • Título:
    Activation of 5-HT 4 receptors facilitates neurogenesis from transplanted neural stem cells in the anastomotic ileum
  • Autor: Goto, Kei ; Kawahara, Isao ; Inada, Hiroyuki ; Misawa, Hiromi ; Kuniyasu, Hiroki ; Nabekura, Junich ; Takaki, Miyako
  • Assuntos: YFP ; 5-HT receptor ; Granulation tissue ; Myenteric neuron ; Neural stem cells
  • É parte de: The Journal of Physiological Sciences, 2016, Vol.66(1), pp.67-76
  • Descrição: An orally administered serotonin-4 (5-HT 4 ) receptor agonist, mosapride citrate (MOS), promotes enteric neurogenesis in anastomoses after gut surgery. We performed gut surgery and transplanted 2 × 10 5 neural stem cells (NSCs) from the embryonic central nervous system after marking them with the cell linker, PKH26. We found that neurons differentiated from transplanted NSCs (PKH [+]) and newborn enteric neurons differentiated from mobilized (host) NSCs (YFP [+]) in the deep granulation tissue of the anastomotic ileum. MOS significantly increased the number of PKH (+) and YFP (+) neurons by 2.5-fold ( P  < 0.005) ( n  = 4). The distribution patterns of PKH (+) neurons and YFP (+) neurons were similar along the depth of the anastomosis. A 5-HT 4 receptor antagonist, SB-207266, abolished these effects of MOS ( n  = 4). Our results indicate that neurogenesis from transplanted NSCs is potentiated by activation of 5-HT 4 receptors. Thus, a combination of drug administration and cell transplantation could be more beneficial than cell transplantation alone in treating Hirschsprung’s disease and related disorders.
  • Idioma: Inglês

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