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The genetic diversity of Epstein–Barr virus in the setting of transplantation relative to non‐transplant settings: A feasibility study

Allen, Upton D. ; Hu, Pingzhao ; Pereira, Sergio L. ; Robinson, Joan L. ; Paton, Tara A. ; Beyene, Joseph ; Khodai‐Booran, Nasser ; Dipchand, Anne ; Hébert, Diane ; Ng, Vicky ; Nalpathamkalam, Thomas ; Read, Stanley

Pediatric Transplantation, February 2016, Vol.20(1), pp.124-129 [Periódico revisado por pares]

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  • Título:
    The genetic diversity of Epstein–Barr virus in the setting of transplantation relative to non‐transplant settings: A feasibility study
  • Autor: Allen, Upton D. ; Hu, Pingzhao ; Pereira, Sergio L. ; Robinson, Joan L. ; Paton, Tara A. ; Beyene, Joseph ; Khodai‐Booran, Nasser ; Dipchand, Anne ; Hébert, Diane ; Ng, Vicky ; Nalpathamkalam, Thomas ; Read, Stanley
  • Assuntos: Epstein–Barr Virus ; Next‐Generation Sequencing ; Genetic Variation ; Whole‐Genome Sequencing
  • É parte de: Pediatric Transplantation, February 2016, Vol.20(1), pp.124-129
  • Descrição: This study examines strains from transplant patients and patients with by sequencing major genes. We also used to detect within total genomic , and to evaluate its genetic variation. Sanger sequencing of major genes was used to compare s from samples taken from transplant patients vs. patients with . We sequenced from a healthy ‐seropositive individual on a HiSeq 2000 instrument. Data were mapped to the reference genomes (876 and B95‐8). The number of 2 s was higher than for 1 and the other genes sequenced within comparable reference coordinates. For 2, there was a median of 15 among transplant samples compared with 10 among samples (p = 0.036). 1 showed little variation between samples. For , we identified 640 and 892 variants at an unadjusted p value of 5 × 10 for 876 and B95‐8 genomes, respectively. We used complementary sequence strategies to examine genetic diversity and its application to transplantation. The results provide the framework for further characterization of strains and related outcomes after organ transplantation.

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