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Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Viana, D. ; Carneiro-Cunha, M. ; Araújo, J. ; Barros-Neto, B. ; Lima-Filho, J. ; Converti, A. ; Pessoa-Júnior, A. ; Porto, A.

Applied Biochemistry and Biotechnology, 2010, Vol.160(6), pp.1797-1807 [Periódico revisado por pares]

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  • Título:
    Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain
  • Autor: Viana, D. ; Carneiro-Cunha, M. ; Araújo, J. ; Barros-Neto, B. ; Lima-Filho, J. ; Converti, A. ; Pessoa-Júnior, A. ; Porto, A.
  • Assuntos: Streptomyces ; Soybean flour ; Glycerol ; Clavulanic acid ; Screening of variables ; Fermentation
  • É parte de: Applied Biochemistry and Biotechnology, 2010, Vol.160(6), pp.1797-1807
  • Descrição: Clavulanic acid (CA) is a β-lactam antibiotic, which has a potent β-lactamase inhibiting activity. The influence of five variables, namely pH (6.0, 6.4, and 6.8), temperature (28°C, 30°C, and 32°C), agitation intensity (150, 200, and 250 rpm), glycerol concentration (5.0, 7.5, and 10 g/L) and soybean flour concentration (5.0, 12.5, and 20 g/L), on CA production by a new isolate of Streptomyces (DAUFPE 3060) was investigated in 250-mL Erlenmeyer flasks using a fractional factorial design. Temperature and soybean flour concentration were shown to be the two variables that exerted the most important effects on the production of CA at 95% confidence level. The highest CA concentration (494 mg/L) was obtained after 48 h at 150 rpm, 32°C, pH 6.0, 5.0 g/L glycerol, and 20 g/L soybean flour concentrations. Under these conditions, the yields of biomass and product on consumed substrate were 0.26 g X /g S and 64.3 mg P /g S , respectively. Fermentations performed in 3.0-L bench-scale fermenter allowed increasing the CA production by about 60%.
  • Idioma: Inglês

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