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Dual Effect of Pyruvate in Isolated Nerve Terminals: Generation of Reactive Oxygen Species and Protection of Aconitase

Tretter, Laszlo ; Liktor, Balint ; Adam-Vizi, Vera

Neurochemical Research, 2005, Vol.30(10), pp.1331-1338 [Periódico revisado por pares]

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  • Título:
    Dual Effect of Pyruvate in Isolated Nerve Terminals: Generation of Reactive Oxygen Species and Protection of Aconitase
  • Autor: Tretter, Laszlo ; Liktor, Balint ; Adam-Vizi, Vera
  • Assuntos: α-ketoglutarate ; α-ketoglutarate dehydrogenase ; aconitase ; Amplex Red ; citrate ; free radicals ; HO ; isocitrate ; mitochondria ; oxidative stress ; pyruvate ; pyruvate dehydrogenase complex ; ROS ; synaptosomes
  • É parte de: Neurochemical Research, 2005, Vol.30(10), pp.1331-1338
  • Descrição: Generation of reactive oxygen species (ROS) in synaptosomes was investigated in the presence of different substrates. When pyruvate was used as a substrate an increased rate of hydrogen peroxide formation was detected by the Amplex Red fluorescent assay, but aconitase, which is known to be a highly sensitive enzyme to ROS was not inhibited. In contrast, pyruvate exerted a partial protection on aconitase against a time-dependent inactivation that occurred when synaptosomes were incubated in the absence of substrates. Disruption of synaptosomal membranes with Triton X-100 prevented the protective effect of pyruvate. It is suggested that citrate and/or isocitrate formed in the metabolism of pyruvate could be responsible for a partial protection of aconitase. Therefore while pyruvate could have a prooxidant effect it could also exert a protective effect on the aconitase.
  • Idioma: Inglês

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