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Stress-mediated alteration in V-ATPase and V-PPase of Butea monosperma

Kumari, Nilima ; Sharma, Vinay

Protoplasma, 2010, Vol.245(1), pp.125-132 [Periódico revisado por pares]

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  • Título:
    Stress-mediated alteration in V-ATPase and V-PPase of Butea monosperma
  • Autor: Kumari, Nilima ; Sharma, Vinay
  • Assuntos: Butea monosperma ; Stress ; V-ATPase ; V-PPase
  • É parte de: Protoplasma, 2010, Vol.245(1), pp.125-132
  • Descrição: The activity and subunit amounts of V-ATPase and V-PPase in various plants of Butea monosperma Taub. (Fabaceae) (ver. Dhak; Palas) growing as a natural inhabitant in varying stress conditions in southeast Rajasthan were studied. Western blot analysis followed by immunological quantification of V-ATPase subunits using specific polyclonal antibodies showed that the subunits A, B, D, E, and c are clearly detectable in all plants, with A, B, and c appearing as intense bands. The other subunits of V-ATPase, viz., C, a, and d, were also detected in majority of the plants. Various subunits exhibited variations in their protein amount in different plants. Besides, a few other clear bands were also detected. Of these, the 30- and 29-kD bands may possibly be Di and Ei. Furthermore, a clear band of V-PPase corresponding to 67–70 kD was also detected. A comparison of the V-ATPase and V-PPase activity revealed that Butea plants in the upper region of the study site showed 70% and 39% higher activity, respectively. Furthermore, the immunological quantification showed that the V-ATPase and V-PPase protein amounts are also higher in the upper Butea plants which have drought stress and, moreover, are also exposed to stronger light intensities for relatively longer duration.
  • Idioma: Inglês

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